Roles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex Crus II in vivo.
Roles of molecular layer interneurons in sensory information processing in mouse cerebellar cortex Crus II in vivo.
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分子层中间神经元在小鼠小脑皮质 Crus II 体内感觉信息处理中的作用
DOI：
10.1371/journal.pone.0037031
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Qiu DL
中科院分区：
文献类型：
--
作者：
Chu CP;Bing YH;Liu H;Qiu DL
小脑皮质分子层中间神经元（MLIs）在小脑皮质对感觉信息的处理中起着关键作用。然而，近期的实验和建模结果对小脑分子层抑制的传统作用提出了质疑。
通过在氨基甲酸乙酯麻醉的ICR小鼠体内全细胞膜片钳记录技术，从小脑皮质 Crus II记录了同侧触须垫喷气刺激诱发的MLIs和浦肯野细胞（PCs）的突触反应。在电流钳（I = 0）模式下，发现喷气刺激主要在PCs中产生抑制作用。在MLIs中，无论是否形成篮状突触连接，这种刺激都会诱发动作电位发放。然而，未形成篮状突触连接的MLIs具有更高的背景活动频率，并且还会产生自发的小尖峰。在电压钳条件下，在MLIs中记录到兴奋性突触后电流（EPSCs），尽管所记录的PCs的主要反应是抑制性突触后电位（IPSP）。所有MLIs的EPSCs潜伏期相似，但EPSCs的时程和幅度随分子层深度而变化。在分子层深部的MLIs中记录到幅度最高、持续时间最短的EPSCs，这些MLIs也形成篮状突触连接。比较MLI和PC的反应，PC的IPSP达到峰值的时间明显比MLI记录到的EPSCs慢。阻断GABAA受体后，在PCs中发现更大的EPSCs，其达到峰值的时间、半峰宽和10 - 90%上升时间也明显比MLIs慢。生物素标记表明MLIs（而非PCs）是染料偶联的。
这些发现表明，面部触觉刺激在小鼠小脑皮质Crus II的MLIs中引起快速兴奋，在PCs中则在较晚的潜伏期产生抑制。这些结果支持了先前的观点，即浦肯野细胞缺乏平行纤维驱动的活动是由于MLI抑制作用所致。
Background Cerebellar cortical molecular layer interneurons (MLIs) play essential roles in sensory information processing by the cerebellar cortex. However, recent experimental and modeling results are questioning traditional roles for molecular layer inhibition in the cerebellum. Methods and Main Results Synaptic responses of MLIs and Purkinje cells (PCs), evoked by air-puff stimulation of the ipsilateral whisker pad were recorded from cerebellar cortex Crus II in urethane-anesthetized ICR mice by in vivo whole-cell patch-clamp recording techniques. Under current-clamp (I = 0), air-puff stimuli were found to primarily produce inhibition in PCs. In MLIs, this stimulus evoked spike firing regardless of whether they made basket-type synaptic connections or not. However, MLIs not making basket-type synaptic connections had higher rates of background activity and also generated spontaneous spike-lets. Under voltage-clamp conditions, excitatory postsynaptic currents (EPSCs) were recorded in MLIs, although the predominant response of recorded PCs was an inhibitory postsynaptic potential (IPSP). The latencies of EPSCs were similar for all MLIs, but the time course and amplitude of EPSCs varied with depth in the molecular layer. The highest amplitude, shortest duration EPSCs were recorded from MLIs deep in the molecular layer, which also made basket-type synaptic connections. Comparing MLI to PC responses, time to peak of PC IPSP was significantly slower than MLI recorded EPSCs. Blocking GABAA receptors uncovered larger EPSCs in PCs whose time to peak, half-width and 10–90% rising time were also significantly slower than in MLIs. Biocytin labeling indicated that the MLIs (but not PCs) are dye-coupled. Conclusions These findings indicate that tactile face stimulation evokes rapid excitation in MLIs and inhibition occurring at later latencies in PCs in mouse cerebellar cortex Crus II. These results support previous suggestions that the lack of parallel fiber driven PC activity is due to the effect of MLI inhibition.
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影响因子：
2.5
作者：
BOWER, JM;WOOLSTON, DC
通讯作者：
WOOLSTON, DC
影响因子：
25
作者：
Loewenstein, Y;Mahon, S;Häusser, MH
通讯作者：
Häusser, MH
影响因子：
4
作者：
Donato, Roberta;Rodrigues, Ricardo J.;Edwards, Frances A.
通讯作者：
Edwards, Frances A.
体内小鼠小脑皮质 Crus II 浦肯野细胞中触觉刺激引起的突触反应
影响因子：
3.7
作者：
Chu CP;Bing YH;Liu QR;Qiu DL
通讯作者：
Qiu DL
影响因子：
5.3
作者：
Hevers, Wulf;Hadley, Stephen H.;Amin, Jahanshah
通讯作者：
Amin, Jahanshah