The Many Potential Fates of Non-Canonical Protein Substrates Subject to NEDDylation.
The Many Potential Fates of Non-Canonical Protein Substrates Subject to NEDDylation.
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DOI：
10.3390/cells10102660
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发表时间：
2021-10-05
期刊：
影响因子：
6
通讯作者：
Dolan BP
中科院分区：
文献类型：
--
作者：
Vijayasimha K;Dolan BP
神经元前体细胞表达的发育下调蛋白8（Neuronal precursor cell-expressed developmentally down-regulated protein 8，NEDD 8）是一种泛素样蛋白（ubiquitin-like protein，UBL），其典型功能包括结合并激活Cullin-Ring finger Ligases（CRL），CRL是真核细胞中最大的泛素连接酶家族之一。然而，近年来，已经鉴定了NEDD 8的几种非典型蛋白质底物。在这里，我们试图审查最近的文献，关于非经典的NEDD化的基板，特别关注如何共价修饰NEDD 8改变蛋白质基板。就像在泛素和UBL的研究中一样，没有明确和全面的解释来满足教科书。在某些情况下，NEDD 8修饰似乎改变底物定位，特别是在应激期间。NEDD化也可能对蛋白质的稳定性产生相互矛盾的影响：一些报告表明NEDD化可以防止降解，而另一些报告表明NEDD化可以促进降解。我们还检查了有多少测量非典型NEDD化的体外研究，并将这些条件与体内可能发生的条件进行比较，例如癌症进展。用于研究非典型NEDD化的条件可能与某些类型的癌症相似，例如胶质母细胞瘤、结肠癌和直肠癌以及肺腺癌。虽然非经典NEDD化的全部结果仍然未知，但我们对文献的回顾表明，研究人员对这种修饰发生的情况保持开放的态度，并确定NEDD 8修饰对他们研究的特定底物的功能影响。
Neuronal precursor cell-expressed developmentally down-regulated protein 8 (NEDD8) is a ubiquitin-like protein (UBL) whose canonical function involves binding to, and thus, activating Cullin–Ring finger Ligases (CRLs), one of the largest family of ubiquitin ligases in the eukaryotic cell. However, in recent years, several non-canonical protein substrates of NEDD8 have been identified. Here we attempt to review the recent literature regarding non-canonical NEDDylation of substrates with a particular focus on how the covalent modification of NEDD8 alters the protein substrate. Like much in the study of ubiquitin and UBLs, there are no clear and all-encompassing explanations to satisfy the textbooks. In some instances, NEDD8 modification appears to alter the substrates localization, particularly during times of stress. NEDDylation may also have conflicting impacts upon a protein’s stability: some reports indicate NEDDylation may protect against degradation whereas others show NEDDylation can promote degradation. We also examine how many of the in vitro studies measuring non-canonical NEDDylation were conducted and compare those conditions to those which may occur in vivo, such as cancer progression. It is likely that the conditions used to study non-canonical NEDDylation are similar to some types of cancers, such as glioblastoma, colon and rectal cancers, and lung adenocarcinomas. Although the full outcomes of non-canonical NEDDylation remain unknown, our review of the literature suggests that researchers keep an open mind to the situations where this modification occurs and determine the functional impacts of NEDD8-modification to the specific substrates which they study.
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影响因子：
64.5
作者：
Duda, David M.;Borg, Laura A.;Scott, Daniel C.;Hunt, Harold W.;Hammel, Michal;Schulman, Brenda A.
通讯作者：
Schulman, Brenda A.
影响因子：
3.7
作者：
Ebina M;Tsuruta F;Katoh MC;Kigoshi Y;Someya A;Chiba T
通讯作者：
Chiba T
DOI：
10.1007/978-981-15-1025-0_6
发表时间：
2020-01-01
期刊：
CULLIN-RING LIGASES AND PROTEIN NEDDYLATION: BIOLOGY AND THERAPEUTICS
影响因子：
--
作者：
Fu, Lin;Cui, Chun-Ping;Zhang, Lingqiang
通讯作者：
Zhang, Lingqiang
针对 Cullin-RING 泛素连接及其在 PROTAC 中的应用。
DOI：
10.1007/978-981-15-1025-0_19
发表时间：
2020-01-01
期刊：
CULLIN-RING LIGASES AND PROTEIN NEDDYLATION: BIOLOGY AND THERAPEUTICS
影响因子：
--
作者：
Gong, Longyuan;Cui, Danrui;Zhao, Yongchao
通讯作者：
Zhao, Yongchao
影响因子：
4
作者：
Chan, Yaru;Yoon, Jeongsook;Chien, Cheng-Ting
通讯作者：
Chien, Cheng-Ting