The effects of aluminum stress on bacterial community diversity in acidic red soils by polymerase chain reaction (PCR)-amplified restriction fragment length polymorphism
The effects of aluminum stress on bacterial community diversity in acidic red soils by polymerase chain reaction (PCR)-amplified restriction fragment length polymorphism
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聚合酶链式反应（PCR）扩增限制性片段长度多态性研究铝胁迫对酸性红壤细菌群落多样性的影响
DOI：
10.5897/ajmr12.347
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发表时间：
2012-04
影响因子：
--
通讯作者：
Wu JC
中科院分区：
文献类型：
--
作者：
He GH;Lin JY;Liu Q;Zhang JF;Wu JC
采用聚合酶链反应-扩增限制性片段长度多态性（PCR-RFLP）技术研究了酸性红壤土壤细菌群落对铝胁迫的响应。在不同铝处理浓度下，从两种典型土壤中共采集到6个细菌群落。提取细菌基因组DNA，经巢式PCR扩增得到16 SrDNA片段，克隆后构建6个16 SrDNA文库。从每个文库中随机选取克隆进行rDNA片段的PCR-RFLP分析，最终得到60个基因型。对这60个基因型进行测序，并通过NCBI的基本局部比对搜索工具（BLAST）（相似性93 - 100%）鉴定其各自的等位基因型，并进行系统发育分析。采用多种多样性指数对克隆库的类型丰富度、频率分布（均匀度）和组成进行了研究。其中，α-、β-、γ-、δ-变形菌属、酸菌属、噬细胞黄杆菌类（CFB组）、绿色非硫菌属、芽单胞菌属、高种污泥属、革兰氏阳性菌属和硝化螺旋菌属占96.7%。序列分析显示，56.7%（34个）的克隆序列与环境中未培养的土壤细菌的序列相似。此外，不同土壤样品中细菌的多样性和组成也比较清楚。A0土样中发现的属数最多，而一些特殊的种，如硝化螺菌，在铝处理的土壤中消失。
The soil bacterial communities in response to aluminum (Al) treatments were evaluated by polymerase chain reaction-amplified restriction fragment length polymorphism (PCR-RFLP) of 16S rRNA genes in acidic red soil samples in this study. A total of 6 bacterial communities were sampled from two representative soil types under different Al-treated concentrations. Bacterial genomic DNA was extracted and nested PCR-amplified to obtain 16S rDNA fragments which were cloned to construct 6 16S rDNA libraries. Clones of each library were selected randomly for PCR-RFLP analysis of rDNA fragments, and eventually 60 genotypes were identified by RFLP fingerprinting. These 60 genotypes were sequenced and their respective phylotype was identified through the Basic Local Alignment Search Tool (BLAST) of NCBI (similarity 93 to 100%) and phylogenetic analyzed. The phylotype richness, frequency distribution (evenness) and composition of the clone libraries were investigated by using a variety of diversity indices. Among these phylotypes, 96.7% belonged to the genera α-, β-, γ-, δProteobacteria, Acidobacteria, Cytophaga-Flavobacteria-Bacteroides (CFB group), green nonsulfur (GNS) bacteria, Gemmatimonadetes, high seed sludge (GC) Gram-positive and Nitrospira. Sequence analyses revealed that 56.7% (34) of clone sequences were similar to those of uncultured soil bacteria in the environment. In addition, the bacterial diversities and compositions were clearly displayed in different soil samples. More genera were discovered in A0 soil sample than any other, and some special species, such as Nitrospira, disappeared in Al-treated soils.
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影响因子：
9.7
作者：
R. Upchurch;C. Chiu;K. Everett;Glen E. Dyszynski;D. Coleman;W. Whitman
通讯作者：
R. Upchurch;C. Chiu;K. Everett;Glen E. Dyszynski;D. Coleman;W. Whitman
影响因子：
4.9
作者：
Meng Ci-fu;Xiaonan Lu;Zhihong Cao;Zhengyi Hu;Wanzhu Ma
通讯作者：
Meng Ci-fu;Xiaonan Lu;Zhihong Cao;Zhengyi Hu;Wanzhu Ma
影响因子：
2.8
作者：
GUIDA, L;SAIDI, Z;POOLE, RK
通讯作者：
POOLE, RK
影响因子：
2.8
作者：
J. Fischer;A. Quentmeier;Sven Gansel;V. Sabadoš;C. Friedrich
通讯作者：
J. Fischer;A. Quentmeier;Sven Gansel;V. Sabadoš;C. Friedrich
影响因子：
4.9
作者：
M. Wood
通讯作者：
M. Wood