Interferon gene expression in sputum cells correlates with the Asthma Index Score during virus-induced exacerbations.
Interferon gene expression in sputum cells correlates with the Asthma Index Score during virus-induced exacerbations.
复制标题
痰细胞中的干扰素基因表达与病毒诱导的加重过程中的哮喘指数评分相关。
DOI：
10.1111/cea.12269
复制
发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
Mathur SK
中科院分区：
文献类型：
--
作者：
Schwantes EA;Manthei DM;Denlinger LC;Evans MD;Gern JE;Jarjour NN;Mathur SK
大多数哮喘加重与病毒性呼吸道感染有关。一些（但不是全部）先前的研究报道，哮喘患者干扰素反应低会增加病毒诱发的急性发作的风险。我们试图确定下气道炎症生物标志物，特别是干扰素基因表达与哮喘患者自然发生的病毒感染的严重程度或病情恶化之间的关系。对一项哮喘加重研究中确诊病毒感染的受试者的痰样本进行分析。监测受试者的日常症状、药物使用和呼气流量峰值直到基线。评估痰样本的细胞计数和基因表达。干扰素γ在哮喘加重患者中的表达明显高于未加重患者（P = 0.002）。IFN‐α1、IFN‐β1和IFN‐γ mRNA水平与哮喘指数峰值相关（r = 0.58, P < 0.001; r = 0.57, P = 0.001; r = 0.51, P = 0.004）。此外，哮喘加重患者IL - 13、IL - 10和嗜酸性粒细胞主要碱性蛋白mRNA水平高于非加重患者（分别为P = 0.03、P = 0.06和P = 0.02）， IL - 13 mRNA与哮喘指数峰值相关（P = 0.006）。我们的研究结果表明，在感染过程的早期，哮喘恶化与干扰素表达的增加而不是减少有关。这些发现提出了一种可能性，即急性感染期间过量的病毒诱导干扰素产生可能导致气道炎症和哮喘恶化。
The majority of asthma exacerbations are related to viral respiratory infections. Some, but not all, previous studies have reported that low interferon responses in patients with asthma increase the risk for virus‐induced exacerbations. We sought to determine the relationship between lower airway inflammatory biomarkers, specifically interferon gene expression, and the severity or presence of an exacerbation in asthmatics experiencing a naturally occurring viral infection. Sputum samples were analysed from subjects in an asthma exacerbation study who experienced a confirmed viral infection. Subjects were monitored for daily symptoms, medication use and peak expiratory flow rate until baseline. Sputum samples were assessed for cell counts and gene expression. Interferon gamma expression was significantly greater in patients with asthma exacerbations compared to non‐exacerbating patients (P = 0.002). IFN‐α1, IFN‐β1 and IFN‐γ mRNA levels correlated with the peak Asthma Index (r = 0.58, P < 0.001; r = 0.57, P = 0.001; and r = 0.51, P = 0.004, respectively). Additionally, IL‐13, IL‐10 and eosinophil major basic protein mRNA levels were greater in patients with asthma exacerbations compared to non‐exacerbating patients (P = 0.03, P = 0.06 and P = 0.02, respectively), and IL‐13 mRNA correlated with the peak Asthma Index (P = 0.006). Our findings indicate that asthma exacerbations are associated with increased rather than decreased expression of interferons early in the course of infection. These findings raise the possibility that excessive virus‐induced interferon production during acute infections can contribute to airway inflammation and exacerbations of asthma.
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DOI：
10.1513/pats.200701-030aw
发表时间：
2007-07-01
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
Johnston, Sebastian L
通讯作者：
Johnston, Sebastian L
影响因子：
6.1
作者：
Gehlhar, K;Bilitewski, C;Bufe, A
通讯作者：
Bufe, A
DOI：
10.1164/rccm.201103-0585oc
发表时间：
2011-11-01
影响因子：
24.7
作者：
Denlinger, Loren C.;Sorkness, Ron L.;Jarjour, Nizar N.
通讯作者：
Jarjour, Nizar N.
DOI：
10.1164/rccm.200407-970oc
发表时间：
2005-03-15
影响因子：
24.7
作者：
Mosser, AG;Vrtis, R;Gern, JE
通讯作者：
Gern, JE
影响因子：
12.4
作者：
Bodey, KJ;Semper, AE;Frew, AJ
通讯作者：
Frew, AJ