Utilization of low-pressure plasma to inactivate bacterial spores on stainless steel screws.
Utilization of low-pressure plasma to inactivate bacterial spores on stainless steel screws.
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利用低压等离子体灭活不锈钢螺丝上的细菌孢子
DOI：
10.1089/ast.2012.0949
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发表时间：
2013
期刊：
影响因子：
4.2
通讯作者：
Moeller R
中科院分区：
文献类型：
--
作者：
Stapelmann K;Fiebrandt M;Raguse M;Awakowicz;Reitz G;Moeller R
行星保护的一个特别重点领域是监测、控制和减少在组装期间和组装后在航天器部件和硬件上检测到的微生物污染。在这项研究中，短小芽孢杆菌SAFR-032（一种持久性航天器组装设施分离株）的野生型孢子和实验室模型生物B。 subtilis168 用于研究低压等离子体（仅使用氢气以及与氧气和蒸发的过氧化氢结合作为工艺气体）对孢子存活的影响，这是通过菌落形成测定来确定的。 B.孢子pumilusSAFR-032 和B。使用无菌技术将枯草杆菌168沉积在不锈钢螺丝的表面上，以模拟孢子污染的航天器硬件组件，随后在甚高频电容耦合等离子体反应器（VHF-CCP）中暴露于不同的等离子体和过氧化氢条件下，以减少孢子负荷。航天器分离的孢子 B. pumilusSAFR-032 对等离子体处理的抵抗力显着高于 B 孢子。枯草芽孢杆菌168。使用低压等离子体并额外处理蒸发的过氧化氢也导致孢子失活增强，其超过单独应用时的任何一种处理，这表明该方法的潜在应用是一种快速且合适的方法，以减少孢子污染的航天器硬件组件，以达到行星保护的目的。关键词：芽孢杆菌—污染—航天器硬件—等离子体灭菌—行星保护。天体生物学 13, 597–606。
A special focus area of planetary protection is the monitoring, control, and reduction of microbial contaminations that are detected on spacecraft components and hardware during and after assembly. In this study, wild-type spores ofBacillus pumilusSAFR-032 (a persistent spacecraft assembly facility isolate) and the laboratory model organismB. subtilis168 were used to study the effects of low-pressure plasma, with hydrogen alone and in combination with oxygen and evaporated hydrogen peroxide as a process gas, on spore survival, which was determined by a colony formation assay. Spores ofB. pumilusSAFR-032 andB. subtilis168 were deposited with an aseptic technique onto the surface of stainless steel screws to simulate a spore-contaminated spacecraft hardware component, and were subsequently exposed to different plasmas and hydrogen peroxide conditions in a very high frequency capacitively coupled plasma reactor (VHF-CCP) to reduce the spore burden. Spores of the spacecraft isolateB. pumilusSAFR-032 were significantly more resistant to plasma treatment than spores ofB. subtilis168. The use of low-pressure plasma with an additional treatment of evaporated hydrogen peroxide also led to an enhanced spore inactivation that surpassed either single treatment when applied alone, which indicates the potential application of this method as a fast and suitable way to reduce spore-contaminated spacecraft hardware components for planetary protection purposes. Key Words:Bacillusspores—Contamination—Spacecraft hardware—Plasma sterilization—Planetary protection. Astrobiology 13, 597–606.
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评估从航天器硬件材料中去除芽孢杆菌孢子的各种清洁方法。
影响因子：
4.2
作者：
K. Venkateswaran;Shirley Y. Chung;J. Allton;R. Kern
通讯作者：
R. Kern
影响因子：
6.9
作者：
Humphreys, P. N.
通讯作者：
Humphreys, P. N.
影响因子：
4.2
作者：
Horneck, Gerda;Moeller, Ralf;Venkateswaran, Kasthuri J.
通讯作者：
Venkateswaran, Kasthuri J.
影响因子：
3.4
作者：
Raballand, V.;Benedikt, J.;von Keudell, A.
通讯作者：
von Keudell, A.
空间微生物学：航天器相关微生物的行星保护、负担、多样性和意义
影响因子：
4.4
作者：
J. Bruckner;S. Osman;K. Venkateswaran;C. Conley
通讯作者：
C. Conley