Cell migration on material-driven fibronectin microenvironments.
Cell migration on material-driven fibronectin microenvironments.
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DOI：
10.1039/c7bm00333a
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发表时间：
2017-06-27
影响因子：
6.6
通讯作者：
Salmeron-Sanchez M
中科院分区：
文献类型：
--
作者：
Grigoriou E;Cantini M;Dalby MJ;Petersen A;Salmeron-Sanchez M
细胞迁移取决于纤维连接蛋白的物理状态，纤维状与球状，这可以通过工程生物材料来控制。细胞迁移是一个基本的过程，涉及广泛的生物现象。然而，控制迁移的基本机制是如何协调的还没有完全理解。在这项工作中，我们探讨了迁移特性的人成纤维细胞使用不同的组织纤连蛋白（FN）触发的两个化学相似的表面，聚（丙烯酸乙酯）（PEA）和聚（丙烯酸甲酯）（PMA）;细胞迁移介导的中间层纤连蛋白（FN）。FN被组织成纳米网络后，简单的吸附在PEA上，而PMA上观察到的球状构象。我们研究了细胞速度超过24小时的过程中，在面积和长度方面的粘着斑的形态。此外，我们分析了细胞分泌的FN的量以及FN重塑。发现人成纤维细胞的速度在PEA上表现出双相行为，而在PMA上保持相当恒定。FA分析显示，随着时间的推移，PEA上的局灶性粘连更成熟，而PMA上的FA较小。最后，人成纤维细胞似乎重塑吸附的FN PMA比PEA。总体而言，这些结果表明，细胞-蛋白质-材料界面影响细胞迁移行为。分析的FA与FN分泌和重塑与细胞速度的差异，提供洞察的因素，可以调节细胞运动。
Cell migration depends on the physical state of fibronectin, fibrillar vs. globular, which can be controlled by engineering biomaterials. Cell migration is a fundamental process involved in a wide range of biological phenomena. However, how the underlying mechanisms that control migration are orchestrated is not fully understood. In this work, we explore the migratory characteristics of human fibroblasts using different organisations of fibronectin (FN) triggered by two chemically similar surfaces, poly(ethyl acrylate) (PEA) and poly(methyl acrylate) (PMA); cell migration is mediated via an intermediate layer of fibronectin (FN). FN is organised into nanonetworks upon simple adsorption on PEA whereas a globular conformation is observed on PMA. We studied cell speed over the course of 24 h and the morphology of focal adhesions in terms of area and length. Additionally, we analysed the amount of cell-secreted FN as well as FN remodelling. Velocity of human fibroblasts was found to exhibit a biphasic behaviour on PEA, whereas it remained fairly constant on PMA. FA analysis revealed more mature focal adhesions on PEA over time contrary to smaller FAs found on PMA. Finally, human fibroblasts seemed to remodel adsorbed FN more on PMA than on PEA. Overall, these results indicate that the cell–protein–material interface affects cell migratory behaviour. Analysis of FAs together with FN secretion and remodelling were associated with differences in cell velocity providing insights into the factors that can modulate cell motility.
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影响因子：
14
作者：
Bergman, AJ;Zygourakis, K
通讯作者：
Zygourakis, K
影响因子：
--
作者：
Berginski ME;Gomez SM
通讯作者：
Gomez SM
DOI：
10.1021/acs.langmuir.5b03259
发表时间：
2016-01-26
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Bathawab F;Bennett M;Cantini M;Reboud J;Dalby MJ;Salmerón-Sánchez M
通讯作者：
Salmerón-Sánchez M
影响因子：
4.6
作者：
Missirlis, Dimitris;Haraszti, Tamas;Spatz, Joachim P.
通讯作者：
Spatz, Joachim P.
影响因子：
4.8
作者：
Kim, Dong-Hwee;Wirtz, Denis
通讯作者：
Wirtz, Denis