A Microbial Electrochemical Technology to Detect and Degrade Organophosphate Pesticides.
A Microbial Electrochemical Technology to Detect and Degrade Organophosphate Pesticides.
复制标题
DOI：
10.1021/acscentsci.1c00931
复制
发表时间：
2021-10-27
影响因子：
18.2
通讯作者：
Furst AL
中科院分区：
文献类型：
--
作者：
Karbelkar AA;Reynolds EE;Ahlmark R;Furst AL
有机磷农药（OP）每年导致数百人患病和死亡。不幸的是，接触往往是通过监测血液和尿液中的降解产物来检测的，在扩散点几乎没有有效的检测和补救方法。我们开发了一种创新的策略来修复这些化合物：一种用于有针对性地检测和销毁OP农药的工程微生物技术。该系统基于使用两种工程菌株的微生物电化学。将菌株组合，使得第一微生物（E. coli）降解农药，而第二种（S. Oneidensis）响应于降解产物产生电流，而不需要外部电化学刺激或标记。这种细胞技术的独特之处在于E。大肠杆菌只是作为酶降解OP的惰性支架，规避了共培养设计的基本要求：同时保持两种微生物菌株的活力。有了这个平台，我们可以检测OP降解产物在亚微摩尔水平，优于报告的比色和荧光传感器。重要的是，这种方法提供了一个模块化的，适应性强的战略，可以扩展到其他环境污染物。我们报告了一种独特的生物传感策略的基础上的混合物工程的S。oneidensis和冻干E.大肠杆菌，同时降解和电化学检测有毒农药。
Organophosphate (OP) pesticides cause hundreds of illnesses and deaths annually. Unfortunately, exposures are often detected by monitoring degradation products in blood and urine, with few effective methods for detection and remediation at the point of dispersal. We have developed an innovative strategy to remediate these compounds: an engineered microbial technology for the targeted detection and destruction of OP pesticides. This system is based upon microbial electrochemistry using two engineered strains. The strains are combined such that the first microbe (E. coli) degrades the pesticide, while the second (S. oneidensis) generates current in response to the degradation product without requiring external electrochemical stimulus or labels. This cellular technology is unique in that the E. coli serves only as an inert scaffold for enzymes to degrade OPs, circumventing a fundamental requirement of coculture design: maintaining the viability of two microbial strains simultaneously. With this platform, we can detect OP degradation products at submicromolar levels, outperforming reported colorimetric and fluorescence sensors. Importantly, this approach affords a modular, adaptable strategy that can be expanded to additional environmental contaminants. We report a unique biosensing strategy based on a mixture of engineered S. oneidensis and freeze-dried E. coli to simultaneously degrade and electrochemically detect toxic pesticides.
登录
查看更多内容
DOI：
10.1016/j.bbrc.2009.10.112
发表时间：
2009-12-25
影响因子：
3.1
作者：
Chen, Baowei;Lei, Chenghong;Shin, Yongsoon;Liu, Jun
通讯作者：
Liu, Jun
影响因子：
8.3
作者：
Heudorf, U;Angerer, J
通讯作者：
Angerer, J
影响因子：
15
作者：
Das, Jagotamoy;Aziz, Md. Abdul;Yang, Haesik
通讯作者：
Yang, Haesik
DOI：
10.3390/s140610432
发表时间：
2014-06-13
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Hayat A;Marty JL
通讯作者：
Marty JL
影响因子：
3.8
作者：
Del Giudice, Immacolata;Coppolecchia, Rossella;Manco, Giuseppe
通讯作者：
Manco, Giuseppe