Clathrin-dependent entry of a gingipain adhesin peptide and Porphyromonas gingivalis into host cells.
Clathrin-dependent entry of a gingipain adhesin peptide and Porphyromonas gingivalis into host cells.
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DOI：
10.1111/j.1462-5822.2008.01228.x
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发表时间：
2008-12
影响因子：
3.4
通讯作者：
Duncan MJ
中科院分区：
文献类型：
--
作者：
Boisvert H;Duncan MJ
牙龈卟啉单胞菌是一种革兰氏阴性的口腔厌氧菌，与牙周炎有关，牙周炎是一种以某种形式影响美国高达80%的成年人口的疾病。该生物体与牙龈上皮和周围组织相互作用，在这项研究中，我们分析了由牙龈卟啉单胞菌和来自表面半胱氨酸蛋白酶家族成员arg-gingipain A的粘附素结构域的肽引发的相互作用。重组肽A44阻断了细菌对宿主细胞单层的粘附，并与细胞膜组分结合。在下拉分析中，A44与参与网格蛋白依赖性内吞途径的蛋白质相关。抑制剂研究证实了网格蛋白的作用，共聚焦显微镜表明，A44包被的珠和完整的细菌与GFP-网格蛋白在宿主细胞中共定位。最后，我们使用siRNA来确定网格蛋白或小窝蛋白-1是否参与肽和完整细菌与宿主细胞的结合。同样，这些测定的结果表明，A44和牙龈卟啉单胞菌两者的结合依赖于网格蛋白的存在，并支持其中A44启动网格蛋白依赖性途径的工作模型，该途径可能导致宿主上皮细胞内化肽或细菌。
Porphyromonas gingivalis, a Gram-negative oral anaerobe, is associated with periodontitis, a disease that in some form affects up to 80% of the adult population in the United States. The organism interacts with gingival epithelium and surrounding tissue, and in this study we analyzed interactions initiated by P. gingivalis and by a peptide derived from the adhesin domain of arg-gingipain A, a member of a family of surface cysteine proteinases. Recombinant peptide A44 blocked adherence of bacteria to host cell monolayers, and bound to components of the cell membrane fraction. In pull-down assays A44 associated with proteins involved in a clathrin-dependent endocytosis pathway. Inhibitor studies confirmed a role for clathrin, and confocal microscopy demonstrated that both A44-coated beads and intact bacteria colocalized with GFP-clathrin in host cells. Finally, we used siRNA to determine whether clathrin or caveolin-1 was involved in association of peptide and intact bacteria with host cells. Again, the results of these assays indicated that association of both A44 and P. gingivalis depended on the presence of clathrin, and support a working model in which A44 initiates a clathrin-dependent pathway that potentially leads to internalization of peptide or bacteria by host epithelial cells.
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影响因子：
3.1
作者：
Hosogi, Y;Duncan, MJ
通讯作者：
Duncan, MJ
影响因子：
3.1
作者：
Hybiske, Kevin;Stephens, Richard S.
通讯作者：
Stephens, Richard S.
影响因子：
3.3
作者：
Contamin, S;Galmiche, A;Boquet, P
通讯作者：
Boquet, P
DOI：
10.1083/jcb.200211018
发表时间：
2003-02-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Abrami L;Liu S;Cosson P;Leppla SH;van der Goot FG
通讯作者：
van der Goot FG
影响因子：
5.4
作者：
Huang, Cheng;Chang, Shin C.;Chang, Ming-Fu
通讯作者：
Chang, Ming-Fu