Barley Anther and Meiocyte Transcriptome Dynamics in Meiotic Prophase I.
Barley Anther and Meiocyte Transcriptome Dynamics in Meiotic Prophase I.
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DOI：
10.3389/fpls.2020.619404
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发表时间：
2020
影响因子：
5.6
通讯作者：
Waugh R
中科院分区：
文献类型：
--
作者：
Barakate A;Orr J;Schreiber M;Colas I;Lewandowska D;McCallum N;Macaulay M;Morris J;Arrieta M;Hedley PE;Ramsay L;Waugh R
在显花植物中，成功的芽细胞发育和减数分裂重组取决于环境和遗传因素的组合。为了深入了解这一专门的生殖发育计划，我们使用了短和长读RNA测序（RNA-seq）来研究免疫细胞学分期的大麦（大麦）花药和性母细胞中转录本丰度的时间动态。我们发现，在花药中最显着的转录变化发生在从减数分裂前到细线期偶线期的过渡，其次是越来越稳定的转录丰度在整个前期I到中期I-四分体。我们的分析表明，减数分裂前的花药中富含长的非编码RNA（lncRNA），进入减数分裂的特征是其强大的和显着的下调。有趣的是，只有24%的推定减数分裂基因直系同源物的集合显示差异转录丰度在至少一个阶段或组织比较。Argonautes，E3泛素连接酶，和lys 48特异性去泛素化酶在前期I性母细胞样品中富集。这些发展，时间分辨的转录组表现出显着的稳定性，在整个前期I在减数分裂进入和早期减数分裂过程中涉及的调控网络的复杂性的初始和实质性重编程后，在母细胞中的转录丰度。
In flowering plants, successful germinal cell development and meiotic recombination depend upon a combination of environmental and genetic factors. To gain insights into this specialized reproductive development program we used short- and long-read RNA-sequencing (RNA-seq) to study the temporal dynamics of transcript abundance in immuno-cytologically staged barley (Hordeum vulgare) anthers and meiocytes. We show that the most significant transcriptional changes in anthers occur at the transition from pre-meiosis to leptotene–zygotene, which is followed by increasingly stable transcript abundance throughout prophase I into metaphase I–tetrad. Our analysis reveals that the pre-meiotic anthers are enriched in long non-coding RNAs (lncRNAs) and that entry to meiosis is characterized by their robust and significant down regulation. Intriguingly, only 24% of a collection of putative meiotic gene orthologs showed differential transcript abundance in at least one stage or tissue comparison. Argonautes, E3 ubiquitin ligases, and lys48 specific de-ubiquitinating enzymes were enriched in prophase I meiocyte samples. These developmental, time-resolved transcriptomes demonstrate remarkable stability in transcript abundance in meiocytes throughout prophase I after the initial and substantial reprogramming at meiosis entry and the complexity of the regulatory networks involved in early meiotic processes.
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影响因子：
3.2
作者：
Naranjo T
通讯作者：
Naranjo T
通过转录组和小 RNA 测序的关联分析深入了解棉花花药发育
影响因子：
5.3
作者：
Chen J;Su P;Chen P;Li Q;Yuan X;Liu Z
通讯作者：
Liu Z
影响因子：
3.7
作者：
Bodi Z;Bottley A;Archer N;May ST;Fray RG
通讯作者：
Fray RG
花芽转录组分析破译了多倍体油菜减数分裂的不规则过程
影响因子：
5.6
作者：
Braynen J;Yang Y;Wei F;Cao G;Shi G;Tian B;Zhang X;Jia H;Wei X;Wei Z
通讯作者：
Wei Z
DOI：
10.1038/nrm4085
发表时间：
2015-12
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Borges F;Martienssen RA
通讯作者：
Martienssen RA