Gut microbiome of helminth-infected indigenous Malaysians is context dependent.
Gut microbiome of helminth-infected indigenous Malaysians is context dependent.
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DOI：
10.1186/s40168-022-01385-x
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发表时间：
2022-12-07
期刊：
影响因子：
15.5
通讯作者：
Lee SC
中科院分区：
文献类型：
--
作者：
Tee MZ;Er YX;Easton AV;Yap NJ;Lee IL;Devlin J;Chen Z;Ng KS;Subramanian P;Angelova A;Oyesola O;Sargsian S;Ngui R;Beiting DP;Boey CCM;Chua KH;Cadwell K;Lim YAL;Loke P;Lee SC
虽然工业化社会的微生物组有很好的特征，但传统生活方式的土著人口的微生物组更类似于古代人类的微生物组。然而，这些人群的宏基因组数据仍然很少，并且与土壤传播的蠕虫感染状况的关系尚不清楚。在这里，我们对来自五个不同寄生虫感染流行率村庄的650个马来西亚土著居民的宏基因组进行了测序。来自蠕虫感染流行率较高的村庄的个体具有更多未绘制的读数和更大的微生物多样性。微生物群落多样性和组成与不同村庄的相关性最强，寄生虫感染状况对微生物组的影响因村庄而异。在蠕虫感染和未感染的个体中，观察到阿苯达唑驱虫药治疗后微生物组的纵向变化。从复制分析的起源推断细菌群体复制率确定了与蠕虫感染相关的特定复制类群。我们的研究结果表明，蠕虫对微生物群的影响高度依赖于环境，阿苯达唑对微生物群的影响可能会混淆驱虫研究的解释。此外，大量的微生物组仍未被注释，这个与蠕虫感染相关的土着人口的大型数据集是未来研究的宝贵资源。视频摘要在线版本包含补充材料，可在10.1186/s40168-022-01385-x获得。
While microbiomes in industrialized societies are well characterized, indigenous populations with traditional lifestyles have microbiomes that are more akin to those of ancient humans. However, metagenomic data in these populations remains scarce, and the association with soil-transmitted helminth infection status is unclear. Here, we sequenced 650 metagenomes of indigenous Malaysians from five villages with different prevalence of helminth infections. Individuals from villages with higher prevalences of helminth infections have more unmapped reads and greater microbial diversity. Microbial community diversity and composition were most strongly associated with different villages and the effects of helminth infection status on the microbiome varies by village. Longitudinal changes in the microbiome in response to albendazole anthelmintic treatment were observed in both helminth infected and uninfected individuals. Inference of bacterial population replication rates from origin of replication analysis identified specific replicating taxa associated with helminth infection. Our results indicate that helminth effects on the microbiota were highly dependent on context, and effects of albendazole on the microbiota can be confounding for the interpretation of deworming studies. Furthermore, a substantial quantity of the microbiome remains unannotated, and this large dataset from an indigenous population associated with helminth infections is a valuable resource for future studies. Video Abstract The online version contains supplementary material available at 10.1186/s40168-022-01385-x.
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影响因子：
7.7
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Beghini F;McIver LJ;Blanco-Míguez A;Dubois L;Asnicar F;Maharjan S;Mailyan A;Manghi P;Scholz M;Thomas AM;Valles-Colomer M;Weingart G;Zhang Y;Zolfo M;Huttenhower C;Franzosa EA;Segata N
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