Functional Characterization of the Pheophytinase Gene, ZjPPH, From Zoysia japonica in Regulating Chlorophyll Degradation and Photosynthesis.
Functional Characterization of the Pheophytinase Gene, ZjPPH, From Zoysia japonica in Regulating Chlorophyll Degradation and Photosynthesis.
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结缕草脱镁叶绿素酶基因 ZjPPH 在调节叶绿素降解和光合作用中的功能特征
DOI：
10.3389/fpls.2021.786570
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发表时间：
2021
影响因子：
5.6
通讯作者：
Wu J
中科院分区：
文献类型：
--
作者：
Teng K;Yue Y;Zhang H;Li H;Xu L;Han C;Fan X;Wu J
叶绿素水解酶（Pheophytinase， PPH）在叶绿素降解中起着重要作用。然而，对暖季禾本科植物的ph值关注较少；迄今为止，它在光合作用中的详细功能都没有被系统地探索过。本研究从结缕草（Zoysia japonica）中分离得到ZjPPH，结缕草是一种优良的暖季草坪草。实时荧光定量PCR和启动子活性分析表明，衰老、ABA和暗诱导均可诱导ZjPPH的表达。亚细胞定位观察证实ZjPPH定位于叶绿体。ZjPPH的过表达加速了叶绿素的降解，挽救了拟南芥pph突变体的留绿表型。此外，ZjPPH通过ABA和可溶性糖含量的积累，以及SAG12和SAG14转录水平的提高，促进了衰老。透射电镜研究表明，ZjPPH引起稳定转化的拟南芥叶绿体超微结构的分解。叶绿素a荧光瞬态测量分析表明，ZjPPH主要通过抑制光系统II （PSII）和光系统I （PSI）抑制光合效率。综上所述，ZjPPH在叶绿素降解和衰老过程中起重要作用。培育具有保持绿色性能和提高光合效率的新种质可能是基因编辑的一个有价值的目标。
Pheophytinase (PPH), the phytol hydrolase, plays important roles in chlorophyll degradation. Nevertheless, little attention has been paid to the PPHs in warm-season grass species; neither its detailed function in photosynthesis has been systematically explored to date. In this study, we isolated ZjPPH from Zoysia japonica, an excellent warm-season turfgrass species. Quantitative real-time PCR analysis and promoter activity characterization revealed that the expression of ZjPPH could be induced by senescence, ABA, and dark induction. Subcellular localization observation proved that ZjPPH was localized in the chloroplasts. Overexpression of ZjPPH accelerated the chlorophyll degradation and rescued the stay-green phenotype of the Arabidopsis pph mutant. Moreover, ZjPPH promoted senescence with the accumulation of ABA and soluble sugar contents, as well as the increased transcriptional level of SAG12 and SAG14. Transmission electron microscopy investigation revealed that ZjPPH caused the decomposition of chloroplasts ultrastructure in stable transformed Arabidopsis. Furthermore, chlorophyll a fluorescence transient measurement analysis suggested that ZjPPH suppressed photosynthesis efficiency by mainly suppressing both photosystem II (PSII) and photosystem I (PSI). In conclusion, ZjPPH plays an important role in chlorophyll degradation and senescence. It could be a valuable target for genetic editing to cultivate new germplasms with stay-green performance and improved photosynthetic efficiency.
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影响因子：
7.4
作者：
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通讯作者：
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影响因子：
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