Nanoparticle vaccines encompassing the respiratory syncytial virus (RSV) G protein CX3C chemokine motif induce robust immunity protecting from challenge and disease.
Nanoparticle vaccines encompassing the respiratory syncytial virus (RSV) G protein CX3C chemokine motif induce robust immunity protecting from challenge and disease.
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DOI：
10.1371/journal.pone.0074905
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Tripp RA
中科院分区：
文献类型：
--
作者：
Jorquera PA;Choi Y;Oakley KE;Powell TJ;Boyd JG;Palath N;Haynes LM;Anderson LJ;Tripp RA
使用逐层制造并掺入包含CX 3C趋化因子基序的呼吸道合胞病毒（RSV）G蛋白多肽来产生纳米颗粒疫苗。用G蛋白纳米颗粒疫苗免疫的BALB/c小鼠在RSV攻击后产生抑制肺中RSV复制的中和抗体应答。ELISPOT分析显示，与RSV攻击后的对照组相比，G纳米颗粒接种小鼠的RSV G蛋白特异性IL-4和IFN-γ分泌细胞水平增加。值得注意的是，与对照相比，G蛋白纳米颗粒疫苗接种的小鼠的RSV攻击导致肺中RSV M2特异性IL-4和IFN-γ分泌T细胞增加，以及M2特异性H-2Kd-四聚体阳性CD 8 + T细胞增加。细胞类型分析显示，接种疫苗与RSV攻毒后肺嗜酸性粒细胞增多无关。这些结果表明，用RSV G蛋白纳米颗粒疫苗接种小鼠诱导有效的中和抗体应答、增加的G蛋白和M2特异性T细胞应答以及RSV疾病发病机制的降低。
Nanoparticle vaccines were produced using layer-by-layer fabrication and incorporating respiratory syncytial virus (RSV) G protein polypeptides comprising the CX3C chemokine motif. BALB/c mice immunized with G protein nanoparticle vaccines produced a neutralizing antibody response that inhibited RSV replication in the lungs following RSV challenge. ELISPOT analysis showed that G nanoparticle vaccinated mice had increased levels of RSV G protein-specific IL-4 and IFN-γ secreting cells compared to controls following RSV challenge. Remarkably, RSV challenge of G protein nanoparticle vaccinated mice resulted in increased RSV M2-specific IL-4 and IFN-γ secreting T cells, and increased M2-specific H-2Kd-tetramer positive CD8+ T cells in the lungs compared to controls. Cell type analysis showed vaccination was not associated with increased pulmonary eosinophilia following RSV challenge. These results demonstrate that vaccination of mice with the RSV G protein nanoparticle vaccines induces a potent neutralizing antibody response, increased G protein- and M2- specific T cell responses, and a reduction in RSV disease pathogenesis.
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DOI：
10.1073/pnas.0806244105
发表时间：
2008-12-30
影响因子：
11.1
作者：
Bueno, Susan M.;Gonzalez, Pablo A.;Kalergis, Alexis M.
通讯作者：
Kalergis, Alexis M.
DOI：
10.1056/nejmoa0804877
发表时间：
2009-02-05
期刊：
The New England journal of medicine
影响因子：
--
作者：
Hall CB;Weinberg GA;Iwane MK;Blumkin AK;Edwards KM;Staat MA;Auinger P;Griffin MR;Poehling KA;Erdman D;Grijalva CG;Zhu Y;Szilagyi P
通讯作者：
Szilagyi P
影响因子：
6.4
作者：
Dowell, SF;Anderson, LJ;Breiman, RF
通讯作者：
Breiman, RF
影响因子：
6.4
作者：
HALL, CB;WALSH, EE;SCHNABEL, KC
通讯作者：
SCHNABEL, KC
影响因子：
15.9
作者：
Gudmundsson, G;Hunninghake, GW
通讯作者：
Hunninghake, GW