The cytoprotective role of taurine in exercise-induced muscle injury
The cytoprotective role of taurine in exercise-induced muscle injury
复制标题
牛磺酸在运动引起的肌肉损伤中的细胞保护作用
作者：
Ralph Dawson;M. Biasetti;S. Messina;J. E. Dominy
总结。剧烈运动被认为会增加氧化应激，损伤肌肉组织。牛磺酸在骨骼肌中存在高浓度，可能在细胞防御自由基介导的损伤中起作用。这项研究的目的是确定控制肌肉中牛磺酸的水平是否会改变运动损伤后自由基损伤的标志物。成年雄性Sprague-Dawley大鼠通过饮用水补充3% （w/v）牛磺酸（n = 10）或竞争性牛磺酸运输抑制剂β-丙氨酸（n = 10），持续一个月。对照组（n = 20）饮用含有0.02%牛磺酸的自来水，所有大鼠均饲喂不含牛磺酸的饮食。除了一组不动的对照组（n = 10）外，所有大鼠都进行了90分钟的下坡跑步机跑步。细胞损伤标志物和自由基损伤标志物随组织氨基酸含量的变化而变化。3%牛磺酸使血浆中牛磺酸水平升高约2倍，3% β-丙氨酸使血浆中牛磺酸水平降低约50%。牛磺酸补充剂（TS）显著提高运动大鼠血浆谷氨酸水平。运动可以降低血浆蛋氨酸水平，而牛磺酸可以防止其下降。补充牛磺酸显著提高了除比目鱼肌外所有肌肉的牛磺酸含量。β-丙氨酸使所有肌肉中牛磺酸含量降低了约50%。脂质过氧化（TBARS）在指长伸肌（EDL）和腓肠肌（GAST）运动中显著增加。牛磺酸和β-丙氨酸完全阻断了EDL中TBARs的增加，但对GAST没有影响。运动可显著降低GAST肌肉的胞浆酶乳酸脱氢酶（LDH）含量，牛磺酸和β-丙氨酸均可减弱这一作用。腓肠肌髓过氧化物酶（MPO）活性显著升高，但饮食对其无影响。肝脏运动显著增加MPO活性，牛磺酸和β-丙氨酸阻断了这一作用。运动或饮食对肺或脾的MPO活性均无影响。通过主观评定量表评估，补充牛磺酸可以改善跑步表现，并且在运动后24小时，服用β-丙氨酸的大鼠体重明显减轻。总之，牛磺酸补充或牛磺酸耗竭具有可测量的细胞保护作用，以减轻运动引起的损伤。
Summary. Intense exercise is thought to increase oxidative stress and damage muscle tissue. Taurine is present in high concentration in skeletal muscle and may play a role in cellular defenses against free radical-mediated damage. The aim of this study was to determine if manipulating muscle levels of taurine would alter markers of free radical damage after exercise-induced injury. Adult male Sprague-Dawley rats were supplemented via the drinking water with either 3% (w/v) taurine (n = 10) or the competitive taurine transport inhibitor, β-alanine (n = 10), for one month. Controls (n = 20) drank tap water containing 0.02% taurine and all rats were placed on a taurine free diet. All the rats except one group of sedentary controls (n = 10) were subjected to 90 minutes of downhill treadmill running. Markers of cellular injury and free radical damage were determined along with tissue amino acid content. The 3% taurine treatment raised plasma levels about 2-fold and 3% β-alanine reduced plasma taurine levels about 50%. Taurine supplementation (TS) significantly increased plasma glutamate levels in exercised rats. Exercise reduced plasma methionine levels and taurine prevented its decline. Taurine supplementation increased muscle taurine content significantly in all muscles except the soleus. β-alanine decreased muscle taurine content about 50% in all the muscles examined. Lipid peroxidation (TBARS) was significantly increased by exercise in the extensor digitorium longus (EDL) and gastrocnemius (GAST) muscles. Both taurine and β-alanine completely blocked the increase in TBARs in the EDL, but had no effect in the GAST. Muscle content of the cytosolic enzyme, lactate dehydrogenase (LDH) was significantly decreased by exercise in the GAST muscle and this effect was attenuated by both taurine and β-alanine. Muscle myeloperoxidase (MPO) activity was significantly elevated in the gastrocnemius muscle, but diet had no effect. MPO activity was significantly increased by exercise in the liver and both taurine and β-alanine blocked this effect. There was no effect of either exercise or the diets on MPO activity in the lung or spleen. Running performance as assessed by a subjective rating scale was improved by taurine supplementation and there was a significant loss in body weight in the β-alanine-treated rats 24 hours after exercise. In summary, taurine supplementation or taurine depletion had measurable cytoprotective actions to attenuate exercise-induced injury.
DOI：
10.1046/j.1525-1373.1999.d01-142.x
发表时间：
1999-12-01
影响因子：
--
作者：
Clanton, TL;Zuo, L;Klawitter, P
通讯作者：
Klawitter, P
影响因子：
3.3
作者：
ARMSTRONG, RB;OGILVIE, RW;SCHWANE, JA
通讯作者：
SCHWANE, JA
影响因子：
4.4
作者：
Liu, Y;Quinn, MR
通讯作者：
Quinn, MR