Biological Evaluation of the Effect of Root Canal Sealers Using a Rat Model.
Biological Evaluation of the Effect of Root Canal Sealers Using a Rat Model.
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DOI：
10.3390/pharmaceutics14102038
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发表时间：
2022-09-24
期刊：
影响因子：
5.4
通讯作者：
Hayashi, Mikako
中科院分区：
文献类型：
--
作者：
Okamoto, Motoki;Matsumoto, Sayako;Moriyama, Kiichi;Huang, Hailing;Watanabe, Masakatsu;Miura, Jiro;Sugiyama, Keita;Hirose, Yujiro;Mizuhira, Manabu;Kuriki, Nanako;Leprince, Julian G.;Takahashi, Yusuke;Kawabata, Shigetada;Hayashi, Mikako
牙胶尖和根管封闭剂在根管治疗中已经使用了几十年。使用诸如单锥方法的技术时，晶体的量更大，使得它们的性质更关键。然而，相对较少的报告全面评价其生物学效应。为此，我们评估了三种类型的密封剂，氧化锌脂肪酸，生物玻璃和甲基丙烯酸酯树脂的密封剂被认为是。采用大鼠皮下埋植模型评价其生物学效应。将每个植入物装入Teflon管内，并皮下植入大鼠背部。在植入后7天，在所有样品周围观察到炎性细胞，并且在28天后减少。我们的结果显示，所有样本均与包皮周围的皮下组织接触。此外，仅在含生物玻璃的玻璃中观察到Ca和P积累。此外，三种封闭剂中的每一种都表现出独特的免疫和炎症调节作用。特别是，发现生物玻璃和甲基丙烯酸酯树脂密封剂诱导与血管生成、伤口愈合、肌肉组织和周围皮下组织相关的相邻皮下组织中的可变基因表达。这些结果可能有助于了解根管封闭剂对周围生物组织的生物学影响，指导未来的研究和与新一代材料的比较。
Gutta-percha points and root canal sealers have been used for decades in endodontics for root canal obturation. With techniques such as single cone methods, the amount of sealer is larger, making their properties more critical. However, relatively few reports have comprehensively evaluated their biological effects. To this end, we evaluated three types of sealers, zinc oxide-fatty acid-, bio-glass- and methacrylate resin-containing sealers were considered. Their biological effects were evaluated using a rat subcutaneous implantation model. Each sealer was loaded inside a Teflon tube and implanted subcutaneously in the backs of rats. Inflammatory cells were observed around all samples 7 days after implantation and reduced after 28 days. Our results revealed that all samples were in contact with the subcutaneous tissue surrounding the sealer. Additionally, Ca and P accumulation was observed in only the bio-glass-containing sealer. Furthermore, each of the three sealers exhibited unique immune and inflammatory modulatory effects. In particular, bio-glass and methacrylate resin sealers were found to induce variable gene expression in adjacent subcutaneous tissues related to angiogenesis, wound healing, muscle tissue, and surrounding subcutaneous tissue. These results may help to understand the biological impacts of root canal sealers on surrounding biological tissues, guiding future research and comparisons with new generations of materials.
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影响因子：
48
作者：
Biancalani T;Scalia G;Buffoni L;Avasthi R;Lu Z;Sanger A;Tokcan N;Vanderburg CR;Segerstolpe Å;Zhang M;Avraham-Davidi I;Vickovic S;Nitzan M;Ma S;Subramanian A;Lipinski M;Buenrostro J;Brown NB;Fanelli D;Zhuang X;Macosko EZ;Regev A
通讯作者：
Regev A
影响因子：
5
作者：
Bouillaguet, S.;Shaw, L.;Wataha, J. C.
通讯作者：
Wataha, J. C.
影响因子：
4.2
作者：
Gaudin, Alexis;Tolar, Mirek;Peters, Ove Andreas
通讯作者：
Peters, Ove Andreas
影响因子：
3.4
作者：
Donnermeyer, David;Bunne, Clarissa;Dammaschke, Till
通讯作者：
Dammaschke, Till
影响因子：
5
作者：
Darvell, Brian W.;Smith, Anthony J.
通讯作者：
Smith, Anthony J.