The Overexpression of NALP3 Inflammasome in Knee Osteoarthritis Is Associated with Synovial Membrane Prolidase and NADPH Oxidase 2.
The Overexpression of NALP3 Inflammasome in Knee Osteoarthritis Is Associated with Synovial Membrane Prolidase and NADPH Oxidase 2.
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DOI：
10.1155/2016/1472567
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发表时间：
2016
影响因子：
--
通讯作者：
López-Reyes A
中科院分区：
文献类型：
--
作者：
Clavijo-Cornejo D;Martínez-Flores K;Silva-Luna K;Martínez-Nava GA;Fernández-Torres J;Zamudio-Cuevas Y;Guadalupe Santamaría-Olmedo M;Granados-Montiel J;Pineda C;López-Reyes A
骨关节炎的特征是存在促炎细胞因子和活性氧。我们的目的是阐明促氧化酶含量在滑膜水平的作用，以及它如何与膝骨关节炎（KOA）患者的炎症过程相关。在KOA患者和对照组的滑膜中，我们分析了促氧化酶如Nox 2、黄嘌呤氧化酶（XO）和脯氨酰二肽酶以及促炎NALP 3的蛋白质含量。结果显示，相对于对照组，氨酰基脯氨酸二肽酶和Nox 2的蛋白质含量分别增加4.8倍和8.4倍，并且XO表现出增加的趋势，而NALP 3炎性体增加5.4倍。氨酰基脯氨酸二肽酶和XO的水平与NALP 3和Nox 2的水平呈正相关。通过主成分分析，对研究组的蛋白质表达模式进行了评价。确定了三个群组;在KOA患者和对照组之间，第二簇（氨酰基脯氨酸二肽酶）和第三簇（XO和Nox 2）的蛋白表达模式较高。数据表明，促氧化酶在膝关节骨性关节炎患者的滑膜增加，并可能有助于炎症状态和关节软骨的降解。
Osteoarthritis is characterized by the presence of proinflammatory cytokines and reactive oxygen species. We aimed to clarify the role of prooxidant enzyme content at the synovial membrane level and how it correlates with the inflammatory process in patients with knee osteoarthritis (KOA). In synovial membranes from KOA patients and control group, we analyzed the protein content of prooxidant enzymes such as Nox2, xanthine oxidase (XO), and prolidase as well as the proinflammatory NALP3. Results show that protein content of prolidase and Nox2 increased 4.8- and 8.4-fold, respectively, and XO showed an increasing trend, while the NALP3 inflammasome increased 5.4-fold with respect to control group. Levels of prolidase and XO had a positive correlation between the levels of NALP3 and Nox2. By principal component analysis the protein expression pattern by study groups was evaluated. Three clusters were identified; protein expression patterns were higher for clusters two (prolidase) and three (XO and Nox2) between KOA patients and controls. Data suggest that prooxidant enzymes increase in synovial membrane of KOA patients and may contribute to the inflammatory state and degradation of the articular cartilage.
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影响因子：
6.1
作者：
Chenevier-Gobeaux, Camille;Simonneau, Catherine;Borderie, Didier
通讯作者：
Borderie, Didier
影响因子：
4.5
作者：
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Song IH
影响因子：
7
作者：
Deberg, M;Labasse, A;Henrotin, Y
通讯作者：
Henrotin, Y
影响因子：
6.4
作者：
Kolly, Laeticia;Busso, Nathalie;So, Alexander
通讯作者：
So, Alexander
DOI：
10.1073/pnas.1111101108
发表时间：
2011-09-06
影响因子：
11.1
作者：
Jin, Chengcheng;Frayssinet, Patrick;Flavell, Richard A.
通讯作者：
Flavell, Richard A.