A Conjugative MDR pMG1-Like Plasmid Carrying the lsa(E) Gene of Enterococcus faecium With Potential Transmission to Staphylococcus aureus.
A Conjugative MDR pMG1-Like Plasmid Carrying the lsa(E) Gene of Enterococcus faecium With Potential Transmission to Staphylococcus aureus.
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携带屎肠球菌 lsa(E) 基因的接合型 MDR pMG1 样质粒，具有向金黄色葡萄球菌传播的潜力
DOI：
10.3389/fmicb.2021.667415
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发表时间：
2021
影响因子：
5.2
通讯作者：
Zhang JZ
中科院分区：
文献类型：
--
作者：
Yan XM;Wang J;Tao XX;Jia HB;Meng FL;Yang H;You YH;Zheng B;Hu Y;Bu XX;Zhang JZ
lsa(E) 是截短侧耳素、林可酰胺和链霉素 A（PLSA 表型）抗性基因，首次在金黄色葡萄球菌中描述，并被认为是从肠球菌属转移而来。本研究旨在阐明三级教学医院屎肠球菌分离株中lsa(E)基因的流行情况，并评估lsa(E)基因在体外从屎肠球菌到金黄色葡萄球菌的可转移性。对2013年北京某医院分离的96株屎肠球菌进行奎奴普丁-达福普丁（QDA）耐药基因分析，并进行多位点序列分型（MLST）。通过过滤交配测定了 10 种屎肠球菌菌株和 4 种金黄色葡萄球菌菌株之间 QDA 抗性的可转移性。对转接合子进行基因组测序。共有 46 株屎肠球菌分离株（46/96，47.92%）lsa（E）检测呈阳性，而 2 株分离株（2/96，2.08%）lsa（A）检测呈阳性。 36株lsa(E)阳性菌株(36/46, 78.3%)属于ST78。在 40 次交配测试中，lsa(E) 通过一次接合成功转移，频率为每个供体 1.125 × 10-7 接合子。转接合子 N7435-R3645 的 QDA 抗性表达水平 (MIC = 16 mg/L) 高于亲本金黄色葡萄球菌菌株 (MIC = 0.38 mg/L)。对转接合子N7435-R3645的二代测序（NGS）分析表明，携带lsa（E）的质粒pN7435-R3645的完整序列大小为92,396 bp，G + C含量为33%（登录号MT022086）。 pN7435-R3645的遗传图谱具有高度的核苷酸相似性，并与两种质粒：屎肠球菌pMG1（AB206333.1）和屎肠球菌LS170308（CP025078.1）共享主要开放阅读框（ORF）特征。 pN7435-R3645的rep基因与pMG1的rep基因表现出100%的同一性，尽管它不属于rep1-19家族，而是属于独特的rep家族。质粒上存在多种抗生素抗性基因，包括lsa(E)、aadE和lnu(B)、erm(B)、ant6-Ia和lnu(B)。总之，鉴定出一种携带 lsa(E) 的质粒，该质粒可以在体外通过接合从屎肠球菌转移到金黄色葡萄球菌。这种多药耐药性 (MDR) pMG1 样质粒可能充当物种间传播抗菌药物耐药性的载体。
lsa(E) is a pleuromutilin, lincosamide, and streptogramin A (PLSA phenotype) resistance gene that was first described in S. aureus and was thought to have been transferred from Enterococcus sp. This study aimed to elucidate the prevalence of the lsa(E) gene among E. faecium isolates at a tertiary teaching hospital and to evaluate the transferability of the lsa(E) gene from E. faecium to S. aureus in vitro. A total of 96 E. faecium strains isolated from one hospital in Beijing in 2013 were analysed for quinupristin-dalfopristin (QDA) resistance genes, and multilocus sequence typing (MLST) was performed. The transferability of QDA resistance between ten E. faecium strains and four S. aureus strains was determined by filter mating. Genome sequencing of the transconjugant was performed. A total of 46 E. faecium isolates (46/96, 47.92%) tested positive for lsa(E), while two isolates (2/96, 2.08%) tested positive for lsa(A). Thirty-six lsa(E)-positive strains (36/46, 78.3%) belonged to ST78. Among 40 mating tests, lsa(E) was successfully transferred through one conjugation at a frequency of 1.125 × 10–7 transconjugants per donor. The QDA resistance of the transconjugant N7435-R3645 was expressed at a higher level (MIC = 16 mg/L) than that of the parent S. aureus strain (MIC = 0.38 mg/L). Next-generation sequencing (NGS) analysis of the transconjugant N7435-R3645 showed that the complete sequence of the lsa(E)-carrying plasmid pN7435-R3645 had a size of 92,396 bp and a G + C content of 33% (accession no. MT022086). The genetic map of pN7435-R3645 had high nucleotide similarity and shared the main open reading frame (ORF) features with two plasmids: E. faecium pMG1 (AB206333.1) and E. faecium LS170308 (CP025078.1). The rep gene of pN7435-R3645 showed 100% identity with that of pMG1, although it did not belong to the rep1-19 family but instead a unique rep family. Multiple antibiotic resistance genes, including lsa(E), aadE and lnu(B), erm(B), ant6-Ia, and lnu(B), were present on the plasmid. In conclusion, an lsa(E)-carrying plasmid that can be transferred by conjugation from E. faecium to S. aureus in vitro was identified. This multidrug resistance (MDR) pMG1-like plasmid may act as a vector in the dissemination of antimicrobial resistance among species.
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猪源耐甲氧西林金黄色葡萄球菌截短侧耳素链霉素A抗性基因lsa(E)的检测及新遗传环境
影响因子：
5.2
作者：
Li, Beibei;Wendlandt, Sarah;Ma, Zhiyong
通讯作者：
Ma, Zhiyong
DOI：
10.1016/j.ijantimicag.2007.08.006
发表时间：
2008-01-01
影响因子：
10.8
作者：
Karanika, M.;Prati, A.;Petinaki, E.
通讯作者：
Petinaki, E.
影响因子：
3.7
作者：
Haghi, Fakhri;Lohrasbi, Vahid;Zeighami, Habib
通讯作者：
Zeighami, Habib
影响因子：
2.6
作者：
de Niederhaeusern, Simona;Bondi, Moreno;Anacarso, Immacolata
通讯作者：
Anacarso, Immacolata
影响因子：
9.4
作者：
Homan, WL;Tribe, D;Willems, RJL
通讯作者：
Willems, RJL