Correlation between Genes of the ceRNA Network and Tumor-Infiltrating Immune Cells and Their Biomarker Screening in Kidney Renal Clear Cell Carcinoma.
Correlation between Genes of the ceRNA Network and Tumor-Infiltrating Immune Cells and Their Biomarker Screening in Kidney Renal Clear Cell Carcinoma.
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DOI：
10.1155/2022/4084461
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发表时间：
2022
影响因子：
--
通讯作者：
Wang, Lixin
中科院分区：
文献类型：
--
作者：
Kong, Aoran;Dong, Hui;Zhang, Guangwen;Qiu, Shuang;Shen, Mengyuan;Niu, Xiaohan;Wang, Lixin
本研究旨在利用生物信息学工具、qPCR和免疫组织化学分析来找出与肾透明细胞癌（KIRC）早期诊断和预后相关的因素。 KIRC 的 lncRNA、miRNA 和 mRNA 的表达谱从癌症基因组图谱数据库下载。基于这三个差异表达基因之间的相互作用构建了ceRNA调控网络。采用CIBERSORT反卷积算法分析22种免疫细胞的差异分布。 Kaplan-Meier 生存和 Cox 分析用于筛选 ceRNA 网络的基因以及与 KIRC 临床和预后预测相关的免疫细胞亚型。在 LINC01426、LINC00894、CCNA2、L1 细胞粘附分子 (L1CAM) 和滤泡辅助 T 细胞之间发现了共表达调控关系，这些细胞可作为潜在的生物标志物。定量逆转录聚合酶链反应结果显示，KIRC中LINC01426表达上调，L1CAM表达下调，但癌组织和癌旁样本中LINC00894和CCNA2的表达水平没有差异。免疫组织化学分析显示，滤泡辅助T细胞更多集中在KIRC的核心组织和转移灶中。总之，LINC01426、L1CAM和滤泡辅助T细胞之间发现了共表达关系，它们可以作为KIRC早期诊断和预后评估的生物标志物。
This study aimed to using bioinformatics tools, qPCR, and the immunohistochemical analysis to find out factors related to the early diagnosis and prognosis of kidney renal clear cell carcinoma (KIRC). The expression profiles of lncRNA, miRNA, and mRNA of KIRC were downloaded from The Cancer Genome Atlas database. A ceRNA regulatory network was constructed based on the interaction between these three differentially expressed genes. The CIBERSORT deconvolution algorithm was used to analyze the differential distribution of 22 types of immune cells. The Kaplan–Meier survival and Cox analyses were used to screen genes of the ceRNA network and also immune cell subtypes related to the clinical and prognostic prediction of KIRC. Co-expression regulatory relationships were found among LINC01426, LINC00894, CCNA2, L1 cell adhesion molecule (L1CAM), and T follicular helper cells, which served as potential biomarkers. The results of quantitative reverse transcriptase-polymerase chain reaction showed that LINC01426 was upregulated while L1CAM was downregulated in KIRC, but no difference was found in the expression levels of LINC00894 and CCNA2 in cancer and adjacent samples. The immunohistochemical analysis showed that T follicular helper cells were more concentrated in core tissues and metastases of KIRC. In a word, co-expression relationships were found among LINC01426, L1CAM, and T follicular helper cells, and they may serve as biomarkers for early diagnosis and prognostic evaluation of KIRC.
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影响因子：
4.6
作者：
Bankhead P;Loughrey MB;Fernández JA;Dombrowski Y;McArt DG;Dunne PD;McQuaid S;Gray RT;Murray LJ;Coleman HG;James JA;Salto-Tellez M;Hamilton PW
通讯作者：
Hamilton PW
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14.9
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DOI：
10.1186/s13046-019-1344-3
发表时间：
2019-08-07
影响因子：
11.3
作者：
Yin, Lei;Li, Wenjia;Peng, Bo
通讯作者：
Peng, Bo
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影响因子：
64.5
作者：
Salmena L;Poliseno L;Tay Y;Kats L;Pandolfi PP
通讯作者：
Pandolfi PP
影响因子：
5.6
作者：
Liang, Feiguo;Liang, Hao;Huang, Peiyuan
通讯作者：
Huang, Peiyuan