DNA aptamer evolved by cell-SELEX for recognition of prostate cancer.
DNA aptamer evolved by cell-SELEX for recognition of prostate cancer.
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Cell-SELEX 进化出的 DNA 适体可识别前列腺癌
DOI：
10.1371/journal.pone.0100243
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Gao X
中科院分区：
文献类型：
--
作者：
Wang Y;Luo Y;Bing T;Chen Z;Lu M;Zhang N;Shangguan D;Gao X
近年来，前列腺癌（PCa）的发病率和死亡率在全球范围内增加。目前现有的诊断和治疗方法并没有使情况得到改善，特别是对于激素难治性前列腺癌（HRPC）。PCa分子探针的缺乏阻碍了PCa转移的早期诊断和准确分期。本研究利用细胞SELEX技术，设计并合成了一种针对前列腺癌细胞系PC-3的核酸适体探针Wy-5a。Wy-5a对靶细胞显示出高特异性，解离常数在纳摩尔范围内，并且不识别其他测试的PCa细胞系和其他测试的肿瘤细胞系。用荧光染料标记的Wy-5a对临床组织切片进行染色显示，来自具有转移的高危组的切片显示出更强的荧光，而来自良性前列腺增生（BPH）的切片没有显示出显著的荧光，这表明适体Wy-5a可能与PCa进展相关的蛋白结合。Wy-5a的高亲和力和特异性使其在前列腺癌的诊断和靶向治疗中具有潜在的应用价值。
Morbidity and mortality of prostate cancer (PCa) have increased in recent years worldwide. Currently existing methods for diagnosis and treatment do not make the situation improve, especially for hormone refractory prostate cancer (HRPC). The lack of molecular probes for PCa hindered the early diagnosis of metastasis and accurate staging for PCa. In this work, we have developed a new aptamer probe Wy-5a against PCa cell line PC-3 by cell-SELEX technique. Wy-5a shows high specificity to the target cells with dissociation constants in the nanomolar range, and does not recognize other tested PCa cell lines and other tested tumor cell lines. The staining of clinical tissue sections with fluorescent dye labeled Wy-5a shows that sections from high risk group with metastasis exhibited stronger fluorescence and sections from Benign Prostatic Hyperplasia (BPH) did not exhibit notable fluorescence, which suggests that aptamer Wy-5a may bind to protein related to the progression of PCa. The high affinity and specificity of Wy-5a makes this aptamer hold potential for application in diagnosis and target therapy of PCa.
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影响因子：
6
作者：
Kovar, Joy L.;Johnson, Mark A.;Simpson, Melanie A.
通讯作者：
Simpson, Melanie A.
影响因子：
7.8
作者：
Osborne, SE;Matsumura, I;Ellington, AD
通讯作者：
Ellington, AD
影响因子：
4.5
作者：
Montironi, R;Mazzuccheli, R;Algaba, F
通讯作者：
Algaba, F
DOI：
10.1056/nejmoa0810696
发表时间：
2009-03-26
期刊：
The New England journal of medicine
影响因子：
--
作者：
Andriole GL;Crawford ED;Grubb RL 3rd;Buys SS;Chia D;Church TR;Fouad MN;Gelmann EP;Kvale PA;Reding DJ;Weissfeld JL;Yokochi LA;O'Brien B;Clapp JD;Rathmell JM;Riley TL;Hayes RB;Kramer BS;Izmirlian G;Miller AB;Pinsky PF;Prorok PC;Gohagan JK;Berg CD;PLCO Project Team
通讯作者：
PLCO Project Team
影响因子：
3.4
作者：
Chen, Hui William;Medley, Colin D.;Tan, Weihong
通讯作者：
Tan, Weihong