Studies of lysozyme binding to histamine as a ligand for hydrophobic charge induction chromatography

Studies of lysozyme binding to histamine as a ligand for hydrophobic charge induction chromatography
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溶菌酶与组胺结合作为疏水电荷诱导色谱配体的研究

DOI:
10.1002/btpr.295
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发表时间:
2009-09
影响因子:
2.9
通讯作者:
Fang-Fang Shen
Fang-Fang Shen
中科院分区:
工程技术4区
文献类型:
--
作者:
Shu Sun;Qing-Hong Shi;Fang-Fang Shen

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将组胺固定在Sepharose CL-6 B(Sepharose)上,用作蛋白质的疏水电荷诱导色谱(HCIC)的配体。采用吸附平衡法和量热法研究了溶菌酶在组胺-琼脂糖凝胶(HA-S)上的吸附,
Histamine was immobilized on Sepharose CL‐6B (Sepharose) for use as a ligand of hydrophobic charge induction chromatography (HCIC) of proteins. Lysozyme adsorption onto Histamine‐Sepharose (HA‐S) was studied by adsorption equilibrium and calorimetry to un
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