Novel serum and urine markers for pediatric appendicitis.
Novel serum and urine markers for pediatric appendicitis.
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DOI：
10.1111/j.1553-2712.2011.01251.x
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发表时间：
2012-01
期刊：
影响因子：
--
通讯作者：
Dayan PS
中科院分区：
文献类型：
--
作者：
Kharbanda AB;Rai AJ;Cosme Y;Liu K;Dayan PS
描述两种新型生物标志物钙卫蛋白和富含亮氨酸的 α 糖蛋白-1 (LRG) 与儿童阑尾炎之间的关联。这是一项前瞻性队列研究，对象为 3 至 18 岁因可能患有阑尾炎而到儿科急诊科就诊的儿童。通过酶联免疫吸附测定法测定血液和尿液样本中的钙卫蛋白和 LRG。最终诊断由组织病理学或电话随访确定。比较患有和不患有阑尾炎的受试者的生物标志物水平。使用递归分区来确定预测阑尾炎的阈值。 176 名受试者中，平均年龄为 11.6 岁（SD ±4.0 岁），其中 52% 为男性。 58 名患者 (34%) 被诊断患有阑尾炎。阑尾炎患者的中位血浆钙卫蛋白、血清 LRG 和尿液 LRG 水平高于非阑尾炎患者 (p < 0.008)。按穿孔状态分层时，非穿孔性阑尾炎的中位血浆钙卫蛋白和血清 LRG 水平高于非穿孔性阑尾炎 (p < 0.01)。与非穿孔性阑尾炎相比，穿孔性阑尾炎的中位血清 LRG、尿液 LRG 和血浆钙卫蛋白水平较高 (p < 0.05)。尿钙卫蛋白在各组之间没有差异。血清 LRG < 40,150 ng/ml、尿液 LRG < 42 ng/ml 和血浆钙卫蛋白 < 159 ng/ml，分别提供 100% 的敏感性和阴性预测值来识别阑尾炎低风险儿童，但特异性范围为 23% 至 35%。标准白细胞 (WBC) 计数达到 100% 的灵敏度，特异性高于两种新型生物标志物。小儿阑尾炎中血浆钙卫蛋白和血清/尿液 LRG 升高。没有任何单个标记的表现与 WBC 一样好。
To describe the association between two novel biomarkers, calprotectin and leucine-rich alpha glycoprotein-1 (LRG), and appendicitis in children. This was a prospective, cohort study of children 3 to 18 years old presenting to a pediatric emergency department with possible appendicitis. Blood and urine samples were assayed for calprotectin and LRG via enzyme-linked immunosorbent assay. Final diagnosis was determined by histopathology or telephone follow-up. Biomarker levels were compared for subjects with and without appendicitis. Recursive partitioning was used to identify thresholds that predicted appendicitis. Of 176 subjects, mean age was 11.6 years (SD ±4.0 years) and 52% were male. Fifty-eight patients (34%) were diagnosed with appendicitis. Median plasma calprotectin, serum LRG, and urine LRG levels were higher in appendicitis versus non-appendicitis (p < 0.008). When stratified by perforation status, median plasma calprotectin and serum LRG levels were higher in non-perforated appendicitis vs. non-appendicitis (p < 0.01). Median serum LRG, urine LRG, and plasma calprotectin levels were higher in perforated appendicitis as compared to non-perforated appendicitis (p < 0.05). Urine calprotectin did not differ among groups. A serum LRG < 40,150 ng/ml, a urine LRG < 42 ng/ml, and a plasma calprotectin < 159 ng/ml, each provided a sensitivity and negative predictive value of 100% to identify children at low risk for appendicitis, but with specificities ranging from 23% to 35%. The standard white blood cell (WBC) count achieved 100% sensitivity at a higher specificity than both novel biomarkers. Plasma calprotectin and serum/urine LRG are elevated in pediatric appendicitis. No individual marker performed as well as the WBC.
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影响因子：
9.6
作者：
Andersson, REB
通讯作者：
Andersson, REB
急性期反应介质在肝细胞中富含亮氨酸α的表达（2） - 糖蛋白的表达。
DOI：
10.1016/j.bbrc.2009.03.104
发表时间：
2009-05-15
影响因子：
3.1
作者：
Shirai R;Hirano F;Ohkura N;Ikeda K;Inoue S
通讯作者：
Inoue S
ELISA用于富含细胞色素c的人血清含有富含浅亮氨酸的α-2-糖蛋白-1作为捕获的配体。
影响因子：
2.2
作者：
Weivoda S;Andersen JD;Skogen A;Schlievert PM;Fontana D;Schacker T;Tuite P;Dubinsky JM;Jemmerson R
通讯作者：
Jemmerson R
影响因子：
120.7
作者：
Flum, DR;Morris, A;Dellinger, EP
通讯作者：
Dellinger, EP
影响因子：
4.4
作者：
Bealer, John F.;Colgin, Mark
通讯作者：
Colgin, Mark