The influence of degradation characteristics of hyaluronic acid hydrogels on in vitro neocartilage formation by mesenchymal stem cells.
The influence of degradation characteristics of hyaluronic acid hydrogels on in vitro neocartilage formation by mesenchymal stem cells.
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DOI：
10.1016/j.biomaterials.2009.04.040
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发表时间：
2009-09
期刊：
影响因子：
14
通讯作者：
Burdick, Jason A.
中科院分区：
文献类型：
--
作者：
Chung, Cindy;Beecham, Michael;Mauck, Robert L.;Burdick, Jason A.
间充质干细胞（MSCs）作为软骨修复的可行细胞来源的潜力取决于能够支持足够软骨组织形成的工程支架的开发。与静态支架相比，动态网络（因交联降解而随时间改变网孔尺寸的水凝胶）可能提供增强整体组织形成所需的调控。在这项研究中，将间充质干细胞光包封在可水解和可酶解的透明质酸（HA）水凝胶组合中，以研究这些水凝胶的可调性以及网络动态变化对新软骨形成的影响。在负载间充质干细胞的透明质酸水凝胶中，当降解与细胞外基质沉积互补时，压缩力学性能提高，而当降解过快时则降低。此外，起始重量百分比更高并降低到较低重量百分比的动态水凝胶不等同于起始为较高或较低重量百分比的静态水凝胶。具体而言，动态变化的2%重量百分比水凝胶（从2%重量百分比降解到1%重量百分比）表现出II型胶原蛋白和聚集蛋白聚糖的上调，并比非动态变化的2%和1%重量百分比水凝胶显示出更高的糖胺聚糖含量。同样，与静态的2%重量百分比和1%重量百分比水凝胶相比，动态系统的力学性能和尺寸维持分别更优。因此，具有动态特性的水凝胶可能改善工程化组织，并有助于将组织工程技术转化为临床应用。
The potential of mesenchymal stem cells (MSCs) as a viable cell source for cartilage repair hinges on the development of engineered scaffolds that support adequate cartilage tissue formation. Evolving networks (hydrogels with mesh sizes that change over time due to crosslink degradation) may provide the control needed to enhance overall tissue formation when compared to static scaffolds. In this study, MSCs were photoencapsulated in combinations of hydrolytically and enzymatically degradable hyaluronic acid (HA) hydrogels to investigate the tunability of these hydrogels and the influence of network evolution on neocartilage formation. In MSC-laden HA hydrogels, compressive mechanical properties increased when degradation complemented extracellular matrix deposition and decreased when degradation was too rapid. In addition, dynamic hydrogels that started at a higher wt% and decreased to a lower wt% were not equivalent to static hydrogels that started at the higher or lower wt%. Specifically, evolving 2wt% hydrogels (2 wt% degrading to 1 wt%) expressed up-regulation of type II collagen and aggrecan, and exhibited increased glycosaminoglycan content over non-evolving 2 and 1 wt% hydrogels. Likewise, mechanical properties and size maintenance were superior in the dynamic system compared to the static 2 wt% and 1 wt% hydrogels, respectively. Thus, hydrogels with dynamic properties may improve engineered tissues and help translate tissue engineering technology to clinical application.
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DOI：
10.1016/j.biocel.2005.08.011
发表时间：
2006-01-01
影响因子：
4
作者：
Iacob, S;Knudson, CB
通讯作者：
Knudson, CB
影响因子：
7.6
作者：
Ohno, S;Ohno-Nakahara, M;Knudson, W
通讯作者：
Knudson, W
影响因子：
2.9
作者：
BITTER, T;MUIR, HM
通讯作者：
MUIR, HM
影响因子：
2.9
作者：
Singer, VL;Jones, LJ;Haugland, RP
通讯作者：
Haugland, RP
DOI：
10.1073/pnas.0737381100
发表时间：
2003-04-29
影响因子：
11.1
作者：
Lutolf, MP;Lauer-Fields, JL;Hubbell, JA
通讯作者：
Hubbell, JA