The Association of Biofilm Formation with Antibiotic Resistance in Lactic Acid Bacteria from Fermented Foods
The Association of Biofilm Formation with Antibiotic Resistance in Lactic Acid Bacteria from Fermented Foods
复制标题
发酵食品中乳酸菌生物膜形成与抗生素耐药性的关系
DOI：
10.1111/jfs.12030
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发表时间：
2013-05
影响因子：
2.4
通讯作者：
Liu, Jingchun
中科院分区：
文献类型：
--
作者：
Zhang, Hongmei;Xie, Lisi;Zhang, Wenyan;Zhou, Wenyuan;Su, Jianyu;Liu, Jingchun
从酸奶和泡菜中分离鉴定了5种细菌的53株乳酸菌。纸片扩散法检测5种抗生素(氨苄西林、红霉素、四环素、万古霉素、链霉素)对所有菌株的敏感性。所有实验室均对链霉素耐药。37株对万古霉素耐药，7株对四环素和红霉素耐药，6株对氨苄西林耐药。用聚合酶链式反应对相应的基因决定簇进行分析。所有耐药株均未检测到耐药基因。用改良的结晶紫染色方法检测生物膜形成能力。53株细菌中，32株具有不同程度的生物被膜形成能力，21株未形成生物被膜。测定了32株可形成生物被膜的菌株的最低抑菌浓度(MIC)和生物被膜抑制浓度(BIC)。除万古霉素外，其余抗生素的生物被膜形成与BIC/MIC值呈显著正相关(P< <； <0.05)。与浮游细菌相比，生物膜表现出更高的耐药性，但不同抗生素对生物膜中抗生素耐药性的提高程度不同。
实际应用
近年来，由于LAB对抗生素的耐药性，对其安全性的研究越来越多。然而，在不同的应用领域，实验室耐药的效果是不同的。因此，有必要全面了解耐药机制。在本研究中，我们发现在药敏试验中表现出抗生素耐药性的实验室分离株没有携带可转移的抗生素耐药性决定簇，而四种抗生素的耐药性与生物被膜形成能力呈显著正相关。本研究得出的数据可作为评价乳酸菌在发酵食品中应用的安全性的依据。
Fifty-three lactic acid bacteria (LAB) strains from five species of bacteria were isolated and identified from yogurt and Chinese pickled vegetables. Susceptibility of all the isolates to five antibiotics (ampicillin, erythromycin, tetracycline, vancomycin, streptomycin) was tested with disc diffusion methods. All LAB were resistant to streptomycin. Thirty-seven strains were resistant to vancomycin, seven strains were resistant to tetracycline and erythromycin, and six strains were resistant to ampicillin. The corresponding genetic determinants were characterized by polymerase chain reaction. No antibiotic resistant gene was detected among all resistant strains. Biofilm-forming ability was monitored by a modified crystal violet staining method. Among the 53 strains, 32 strains showed different levels of biofilm-forming ability, while 21 strains did not form biofilms. The minimal inhibitory concentration of antibiotics (MIC) and the biofilm inhibitory concentration (BIC) were determined in the 32 strains which can form biofilms. There are significance correlations between biofilm formation and BIC/MIC for all antibiotics (P < 0.05) except vancomycin. Biofilms exhibited increased antimicrobial resistance compared with planktonic isolates, but the extent of enhanced antibiotic resistance in biofilms is different with different antibiotics.
Practical Application
Recently, there are increasing researches focusing on the safety of LAB, due to their antibiotic resistance. However, effects of antibiotic resistance in LAB were different in different application fields. So it is necessary to understand the mechanism of resistance all sidedly. In this study, we found those LAB isolates, which exhibiting antibiotics resistance in the susceptive tests, carried no transferable antibiotic resistance determinant, while there was a significant positive correlation between four antibiotics resistance and biofilm-forming ability. Data derived from this study can be used as a basis for assessing the safety of LAB applied in fermented food.
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影响因子：
3.3
作者：
Ng, LK;Martin, I;Mulvey, M
通讯作者：
Mulvey, M
DOI：
10.1007/978-94-011-1824-8_4
发表时间：
1992
期刊：
--
影响因子：
--
作者：
J. Holah;L. Kearney
通讯作者：
J. Holah;L. Kearney
影响因子：
2.6
作者：
M. S. Sidhu;S. Langsrud;A. Holck
通讯作者：
M. S. Sidhu;S. Langsrud;A. Holck
影响因子：
5.3
作者：
A. Singh;A. Ramesh
通讯作者：
A. Singh;A. Ramesh
影响因子：
4.4
作者：
Franz, CMAP;Muscholl-Silberhorn, AB;Holzapfel, WH
通讯作者：
Holzapfel, WH