A quantitative assessment of nanoparticle-ligand distributions: implications for targeted drug and imaging delivery in dendrimer conjugates.
A quantitative assessment of nanoparticle-ligand distributions: implications for targeted drug and imaging delivery in dendrimer conjugates.
复制标题
DOI：
10.1021/nn900999c
复制
发表时间：
2010-02-23
期刊：
影响因子：
17.1
通讯作者：
Banaszak Holl MM
中科院分区：
文献类型：
--
作者：
Mullen DG;Fang M;Desai A;Baker JR;Orr BG;Banaszak Holl MM
功能纳米粒子通常含有配体，包括靶向分子、荧光团和/或活性部分（例如药物）。表征与每个颗粒结合的配体的数量以及纳米颗粒配体种类的分布对于理解纳米材料的功能非常重要。在本研究中，检查了通常用于将配体与聚（酰胺胺）（PAMAM）树枝状聚合物缀合的酰胺偶联方法。使用 HPLC 观察并量化了两组树枝状聚合物-配体样品的偏态泊松分布，这些样品是使用胺封端形式的 PAMAM 树枝状聚合物和部分乙酰化形式的 PAMAM 树枝状聚合物（已用于体内靶向药物递送）制备的。制备的样品中每个树枝状聚合物的平均配体数量为 0.4 至 13 个。HPLC 鉴定的分布与通过 1 H NMR、凝胶渗透色谱 (GPC) 和电位滴定测量的平均配体/树枝状聚合物比率非常一致。这些结果提供了对配体缀合的许多类别纳米材料所获得的分布异质性的深入了解，并掩盖了简单卡通模型的使用，该模型将结合的配体的“平均”数量呈现为材料的物理意义表示。
Functional nanoparticles often contain ligands including targeting molecules, fluorophores, and/or active moieties such as drugs. Characterizing the number of these ligands bound to each particle, and the distribution of nanoparticle-ligand species, is important for understanding the nanomaterial’s function. In this study, the amide coupling methods commonly used to conjugate ligands to poly(amidoamine) (PAMAM) dendrimers were examined. A skewed Poisson distribution was observed and quantified using HPLC for two sets of dendrimer-ligand samples prepared using the amine terminated form of the PAMAM dendrimer and a partially acetylated form of the PAMAM dendrimer that has been used for targeted in vivo drug delivery. The prepared samples had an average number of ligands per dendrimer ranging from 0.4 to 13. Distributions identified by HPLC are in excellent agreement with the mean ligand/dendrimer ratio, measured by 1H NMR, gel permeation chromatography (GPC), and potentiometric titration. These results provide insight into the heterogeneity of distributions that are obtained for many classes of nanomaterials to which ligands are conjugated and belie the use of simple cartoon models that present the “average” number of ligands bound as a physically meaningful representation for the material.
登录
查看更多内容
影响因子：
1.8
作者：
Giordanengo, Remi;Mazarin, Michael;Charles, Laurence
通讯作者：
Charles, Laurence
影响因子：
7.3
作者：
KRALOVEC, J;SPENCER, G;GHOSE, T
通讯作者：
GHOSE, T
影响因子：
6.2
作者：
Majoros, IJ;Myc, A;Baker, JR
通讯作者：
Baker, JR
影响因子：
4.9
作者：
Chandrasekar, Durairaj;Sistla, Ramakrishna;Diwan, Prakash V.
通讯作者：
Diwan, Prakash V.
影响因子：
5.5
作者：
Majoros, IJ;Keszler, B;Baker, JR
通讯作者：
Baker, JR