Intricate genetic variation networks control the adventitious root growth angle in apple.
Intricate genetic variation networks control the adventitious root growth angle in apple.
复制标题
复杂的遗传变异网络控制着苹果不定根的生长角度。
DOI：
10.1186/s12864-020-07257-8
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发表时间：
2020-12-01
期刊：
影响因子：
4.4
通讯作者：
Han Z
中科院分区：
文献类型：
--
作者：
Zheng C;Shen F;Wang Y;Wu T;Xu X;Zhang X;Han Z
根生长角（RGA）通常决定植物生根深度，这对于植物锚定和非生物胁迫耐受性具有重要意义。已在作物中鉴定出几个 RGA 数量性状基因座 (QTL)。然而，人们对 RGA 的基本机制仍知之甚少，特别是在苹果砧木中。本研究的目的是鉴定 QTL、验证遗传变异网络并开发苹果砧木 RGA 的分子标记。通过测序进行批量分离分析 (BSA-seq)，使用 1955 年苹果砧木品种“Baleng Crab”（MalusRobusta Rehd.，大 RGA）和“M9”（M. pumila Mill.，小 RGA）的杂交体鉴定了 25 个 RGA QTL。通过RNA测序（RNA-seq）和亲本重测序，鉴定了6个主要功能基因，并构成了RGA的两个遗传变异网络。 MdLAZY1启动子的两个单核苷酸多态性（SNP）损坏了MdDREB2A和MdHSFB3的结合位点，而MdDREB2A和MdIAA1的一个SNP分别影响了MdDREB2A/MdHSFB3和MdIAA1/MdLAZY1的相互作用。 MdNPR5启动子内的一个SNP破坏了MdNPR5和MdLBD41之间的相互作用，而MdLBD41的一个SNP中断了MdLBD41/MdbHLH48相互作用，从而影响了MdLBD41与MdNPR5启动子的结合能力。每个QTL区间的候选基因设计了26个SNP标记，标记效应变化范围为0.22°-26.11°。六个诊断标记（SNP592、G122、b13、Z312、S1272 和 S1288）用于识别控制 RGA 的两个复杂的遗传变异网络，并可能为分子标记的准确性提供新的见解。 QTL 和 SNP 标记可用于选择深根苹果砧木。
The root growth angle (RGA) typically determines plant rooting depth, which is significant for plant anchorage and abiotic stress tolerance. Several quantitative trait loci (QTLs) for RGA have been identified in crops. However, the underlying mechanisms of the RGA remain poorly understood, especially in apple rootstocks. The objective of this study was to identify QTLs, validate genetic variation networks, and develop molecular markers for the RGA in apple rootstock. Bulked segregant analysis by sequencing (BSA-seq) identified 25 QTLs for RGA using 1955 hybrids of the apple rootstock cultivars ‘Baleng Crab’ (Malus robusta Rehd., large RGA) and ‘M9’ (M. pumila Mill., small RGA). With RNA sequencing (RNA-seq) and parental resequencing, six major functional genes were identified and constituted two genetic variation networks for the RGA. Two single nucleotide polymorphisms (SNPs) of the MdLAZY1 promoter damaged the binding sites of MdDREB2A and MdHSFB3, while one SNP of MdDREB2A and MdIAA1 affected the interactions of MdDREB2A/MdHSFB3 and MdIAA1/MdLAZY1, respectively. A SNP within the MdNPR5 promoter damaged the interaction between MdNPR5 and MdLBD41, while one SNP of MdLBD41 interrupted the MdLBD41/MdbHLH48 interaction that affected the binding ability of MdLBD41 on the MdNPR5 promoter. Twenty six SNP markers were designed on candidate genes in each QTL interval, and the marker effects varied from 0.22°-26.11°. Six diagnostic markers, SNP592, G122, b13, Z312, S1272, and S1288, were used to identify two intricate genetic variation networks that control the RGA and may provide new insights into the accuracy of the molecular markers. The QTLs and SNP markers can potentially be used to select deep-rooted apple rootstocks.
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
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