Gene expression profiling in juvenile and mature cuttings of Eucalyptus grandis reveals the importance of microtubule remodeling during adventitious root formation.
Gene expression profiling in juvenile and mature cuttings of Eucalyptus grandis reveals the importance of microtubule remodeling during adventitious root formation.
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DOI：
10.1186/1471-2164-15-826
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发表时间：
2014-09-30
期刊：
影响因子：
4.4
通讯作者：
Sadot E
中科院分区：
文献类型：
--
作者：
Abu-Abied M;Szwerdszarf D;Mordehaev I;Yaniv Y;Levinkron S;Rubinstein M;Riov J;Ophir R;Sadot E
不定根形成能力是木本植物从幼龄到成熟阶段转变过程中丧失的重要经济性状。生长素处理，通常促进生根在幼年插条，往往是无效的，当应用于成熟插条。巨桉中这种现象的分子基础在这里解决。一个全面的微阵列分析，以比较基因表达谱在幼年和成熟的扦插E。在AR诱导后的第0、1、3、6、9和12天，用或不用生长素处理，对grandis进行处理。在这些条件下，AR原基只形成在生长素处理的幼穗。然而，聚类的表达谱显示，诱导后的时间比插条或生长素处理的发育阶段更显着地贡献了表达的差异。大多数检测到的差异，这是有关的发展阶段和生长素治疗发生在第6天，这与AR-原基形成的动力学。少年和成熟的插条之间的转录本的功能组不同的是微管（MT）。在相同的RNA样品中验证了注释为编码微管蛋白、MT相关蛋白和驱动蛋白马达蛋白的42个转录物的表达。这些结果表明，协调发展和生长素依赖的几个MT相关的转录调控在这些插条。为了确定MT重塑与AR形成的相关性，MT受到在生长素诱导期间应用的MT破坏药物氟乐灵的微妙扰动。幼穗不受处理的影响，但成熟的插穗生根从10%增加到40%以上。这些数据表明幼年特异性MT重塑参与了E.奶奶。本文的在线版本（doi：10.1186/1471-2164-15-826）包含补充材料，可供授权用户使用。
The ability to form adventitious roots (AR) is an economically important trait that is lost during the juvenile-to-mature phase change in woody plants. Auxin treatment, which generally promotes rooting in juvenile cuttings, is often ineffective when applied to mature cuttings. The molecular basis for this phenomenon in Eucalyptus grandis was addressed here. A comprehensive microarray analysis was performed in order to compare gene-expression profiles in juvenile and mature cuttings of E. grandis, with or without auxin treatment on days, 0, 1, 3, 6, 9 and 12 post AR induction. Under these conditions AR primordia were formed only in auxin-treated juvenile cuttings. However, clustering the expression profiles revealed that the time after induction contributed more significantly to the differences in expression than the developmental phase of the cuttings or auxin treatment. Most detected differences which were related to the developmental phase and auxin treatment occurred on day 6, which correlated with the kinetics of AR-primordia formation. Among the functional groups of transcripts that differed between juvenile and mature cuttings was that of microtubules (MT). The expression of 42 transcripts annotated as coding for tubulin, MT-associated proteins and kinesin motor proteins was validated in the same RNA samples. The results suggest a coordinated developmental and auxin dependent regulation of several MT-related transcripts in these cuttings. To determine the relevance of MT remodeling to AR formation, MTs were subjected to subtle perturbations by trifluralin, a MT disrupting drug, applied during auxin induction. Juvenile cuttings were not affected by the treatment, but rooting of mature cuttings increased from 10 to more than 40 percent. The data suggest that juvenile-specific MT remodeling is involved in AR formation in E. grandis. The online version of this article (doi:10.1186/1471-2164-15-826) contains supplementary material, which is available to authorized users.
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影响因子：
1.1
作者：
PATON, DM;WILLING, RR;PRYOR, LD
通讯作者：
PRYOR, LD
影响因子：
7.4
作者：
Brinker, M;van Zyl, L;von Arnold, S
通讯作者：
von Arnold, S
影响因子：
4.2
作者：
GREENWOOD, MS
通讯作者：
GREENWOOD, MS
影响因子：
6.4
作者：
Díaz-Sala, C;Garrido, G;Sabater, B
通讯作者：
Sabater, B
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y