Human leukocyte telomere length is associated with DNA methylation levels in multiple subtelomeric and imprinted loci.
Human leukocyte telomere length is associated with DNA methylation levels in multiple subtelomeric and imprinted loci.
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DOI：
10.1038/srep04954
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发表时间：
2014-05-14
影响因子：
4.6
通讯作者：
Pembrey M
中科院分区：
文献类型：
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作者：
Buxton JL;Suderman M;Pappas JJ;Borghol N;McArdle W;Blakemore AI;Hertzman C;Power C;Szyf M;Pembrey M
在人类中，白细胞端粒长度（LTL）与寿命呈正相关，较短的LTL与年龄相关疾病的风险增加有关。在这项研究中，我们测试了端粒长度和甲基化胞嘧啶水平之间的关联。从44-45岁的男性的血液（N = 24）和EBV转化的细胞系（N = 36）DNA样品中获得> 450，000个CpG位点处的平均端粒长度和DNA甲基化的测量。我们鉴定了65个富含甲基化水平与白细胞端粒长度相关的CpG位点的基因启动子，以及36个富含甲基化水平与EBV转化细胞系DNA端粒长度相关的CpG位点的基因启动子。我们观察到端粒下基因座（端粒4 Mb内）（P < 0.01）以及印记区域基因座（P < 0.001）中正相关的甲基化CpG位点显着富集。 我们的研究结果为进一步研究铺平了道路，有助于阐明健康和疾病中端粒长度，DNA甲基化和基因表达之间的关系。
In humans, leukocyte telomere length (LTL) is positively correlated with lifespan, and shorter LTL is associated with increased risk of age-related disease. In this study we tested for association between telomere length and methylated cytosine levels. Measurements of mean telomere length and DNA methylation at >450,000 CpG sites were obtained for both blood (N = 24) and EBV-transformed cell-line (N = 36) DNA samples from men aged 44–45 years. We identified 65 gene promoters enriched for CpG sites at which methylation levels are associated with leukocyte telomere length, and 36 gene promoters enriched for CpG sites at which methylation levels are associated with telomere length in DNA from EBV-transformed cell-lines. We observed significant enrichment of positively associated methylated CpG sites in subtelomeric loci (within 4 Mb of the telomere) (P < 0.01), and also at loci in imprinted regions (P < 0.001). Our results pave the way for further investigations to help elucidate the relationships between telomere length, DNA methylation and gene expression in health and disease.
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影响因子：
3.7
作者：
Farzaneh-Far R;Lin J;Epel E;Lapham K;Blackburn E;Whooley MA
通讯作者：
Whooley MA
影响因子：
4.4
作者：
Bibikova, Marina;Barnes, Bret;Shen, Richard
通讯作者：
Shen, Richard
全基因组甲基化谱揭示了人类衰老速度的定量观点。
影响因子：
16
作者：
Hannum, Gregory;Guinney, Justin;Zhao, Ling;Zhang, Li;Hughes, Guy;Sadda, SriniVas;Klotzle, Brandy;Bibikova, Marina;Fan, Jian-Bing;Gao, Yuan;Deconde, Rob;Chen, Menzies;Rajapakse, Indika;Friend, Stephen;Ideker, Trey;Zhang, Kang
通讯作者：
Zhang, Kang
影响因子：
14.9
作者：
Cawthon RM
通讯作者：
Cawthon RM
DOI：
10.1038/nrg3246
发表时间：
2012-10
期刊：
Nature reviews. Genetics
影响因子：
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作者：
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