Evolved polygenic herbicide resistance in Lolium rigidum by low-dose herbicide selection within standing genetic variation.
Evolved polygenic herbicide resistance in Lolium rigidum by low-dose herbicide selection within standing genetic variation.
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DOI：
10.1111/j.1752-4571.2012.00282.x
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发表时间：
2013-02
影响因子：
4.1
通讯作者：
Powles S
中科院分区：
文献类型：
--
作者：
Busi R;Neve P;Powles S
选择条件下环境与遗传性状的相互作用是进化的基础。在这项研究中，我们已经调查了除草剂抗性的遗传基础，在一个高度特征化的初始除草剂敏感的硬直黑麦草人口反复选择低（低于推荐标签）剂量的除草剂禾草灵。我们报告了在F1代家庭中观察到的除草剂抗性水平的变异性和在F2代和回交（BC）家庭中观察到的抗性分离。所选择的除草剂抗性表型性状似乎处于复杂的多基因控制下。根据除草剂剂量的不同，对有效最小基因数（NE）的估计表明，至少有三个抗性基因得到了富集。联合尺度检验表明，加性-显性模型最能解释亲本、F1、F2和BC家族中的基因互作。孟德尔研究6个F2和两个BC分离的家庭证实参与一个以上的抗性基因。异花传粉L.在低剂量选择条件下，硬粒小麦可通过小效应加性基因的数量积累，迅速进化出多基因广谱抗性。这可以通过以推荐剂量使用除草剂来最小化，这会导致高死亡率，超出除草剂敏感性表型变异的正常范围。
The interaction between environment and genetic traits under selection is the basis of evolution. In this study, we have investigated the genetic basis of herbicide resistance in a highly characterized initially herbicide-susceptible Lolium rigidum population recurrently selected with low (below recommended label) doses of the herbicide diclofop-methyl. We report the variability in herbicide resistance levels observed in F1 families and the segregation of resistance observed in F2 and back-cross (BC) families. The selected herbicide resistance phenotypic trait(s) appear to be under complex polygenic control. The estimation of the effective minimum number of genes (NE), depending on the herbicide dose used, reveals at least three resistance genes had been enriched. A joint scaling test indicates that an additive-dominance model best explains gene interactions in parental, F1, F2 and BC families. The Mendelian study of six F2 and two BC segregating families confirmed involvement of more than one resistance gene. Cross-pollinated L. rigidum under selection at low herbicide dose can rapidly evolve polygenic broad-spectrum herbicide resistance by quantitative accumulation of additive genes of small effect. This can be minimized by using herbicides at the recommended dose which causes high mortality acting outside the normal range of phenotypic variation for herbicide susceptibility.
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影响因子：
5.8
作者：
Podlich, DW;Cooper, M
通讯作者：
Cooper, M
影响因子：
4.3
作者：
Brotherton, Jeffrey E.;Jeschke, Mark R.;Widholm, Jack M.
通讯作者：
Widholm, Jack M.
影响因子：
4.1
作者：
Gomulkiewicz, Richard;Holt, Robert D.;Nuismer, Scott L.
通讯作者：
Nuismer, Scott L.
影响因子：
7.3
作者：
Busi, Roberto;Powles, Stephen B.
通讯作者：
Powles, Stephen B.
影响因子：
1.7
作者：
Onofri, A.;Carbonell, E. A.;Cousens, R. D.
通讯作者：
Cousens, R. D.