Studies on antimicrobial peptide-loaded nanomaterial for root caries restorations to inhibit periodontitis related pathogens in periodontitis care
Studies on antimicrobial peptide-loaded nanomaterial for root caries restorations to inhibit periodontitis related pathogens in periodontitis care
复制标题
牙周炎护理中载抗菌肽纳米材料用于根面龋修复抑制牙周炎相关病原体的研究
DOI：
10.1080/02652048.2020.1842528
复制
发表时间：
2020-11
影响因子：
3.9
通讯作者：
Wu Buling
中科院分区：
文献类型：
--
作者：
Hu Yuqian;Chen Yan;Lin Lijuan;Zhang Jinhui;Lan Rengang;Wu Buling
摘要目的制备一种新型抗菌肽Nal-P-113-PEG-CSNPs，用于根面龋的防治，以控制牙周炎相关病原菌的感染。方法通过简单的聚合方法制备纳米粒，并使用有效的分析方法（TEM，UV等）进行表征。通过不同的体外方法测试Nal-P-113-PEG-CSNPs对牙周细菌病原体的抗菌活性和生物膜形成。结果纳米粒的粒径为216.2 ± 1.6nm。发现Nal-P-113-PEG-CSNP的药物包封效率（%EE（w/w））为89.33 ± 1.67%（w/w）。抑菌试验表明，纳米粒对具核梭杆菌、戈登链球菌和牙龈卟啉单胞菌的生长有较好的抑制作用，MIC分别为23 μg/mL、6 μg/mL和31 μg/mL。结论制备的抗菌肽PEG-CSNPs具有良好的体外抗菌活性，但其对牙周炎的治疗效果有待进一步研究。
Abstract Aims To prepare a novel antimicrobial peptide Nal-P-113 loaded poly (ethylene glycol) combined chitosan nanoparticles (Nal-P-113-PEG-CSNPs) for root caries restorations to control the periodontitis related pathogens in periodontitis care. Methods Nanoparticles were prepared by simple polymerisation method and characterised using effective analytical methods (TEM, UV, etc.). The antimicrobial activity and biofilm formation of Nal-P-113-PEG-CSNPs was tested against periodontal bacterial pathogens by different in vitro methods. Results The size of Nal-P-113 loaded PEG-Chitosn nanoparticles was 216.2 ± 1.6 nm. The drug encapsulation efficiency (%EE (w/w) of Nal-P-113-PEG-CSNPs was found to be 89.33 ± 1.67% (w/w). The antimicrobial examination showed that prepared NPs have effectively inhibited the growth of Fusobacterium nucleatum, Streptococcus gordonii, and Porphyromonas gingivalis with the MIC of 23 µg/mL, 6 µg/mL and 31 µg/mL, respectively. Conclusions The prepared antimicrobial peptide-loaded PEG-CSNPs provide excellent in vitro efficiency but, further studies are necessary to confirm its therapeutic efficacy on periodontitis care.
登录
查看更多内容
影响因子：
2.1
作者：
Suzuki N;Yoneda M;Hirofuji T
通讯作者：
Hirofuji T
影响因子：
6.7
作者：
Hugoson, Anders;Sjodin, Bengt;Norderyd, Ola
通讯作者：
Norderyd, Ola
DOI：
--
发表时间：
2017-04
期刊：
International Journal of Health Sciences
影响因子：
--
作者：
M. Nazir
通讯作者：
M. Nazir
影响因子：
3.1
作者：
Pem, Barbara;Pongrac, Igor M.;Vrcek, Ivana Vinkovic
通讯作者：
Vrcek, Ivana Vinkovic
DOI：
10.14219/jada.archive.2009.0356
发表时间：
2009-09-01
影响因子：
3.9
作者：
Armitage, Gary C.;Robertson, Paul B.
通讯作者：
Robertson, Paul B.