Histological and biochemical analysis of mechanical and thermal bioeffects in boiling histotripsy lesions induced by high intensity focused ultrasound.
Histological and biochemical analysis of mechanical and thermal bioeffects in boiling histotripsy lesions induced by high intensity focused ultrasound.
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DOI：
10.1016/j.ultrasmedbio.2012.10.012
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发表时间：
2013-03
影响因子：
2.9
通讯作者：
Khokhlova, Vera
中科院分区：
文献类型：
--
作者：
Wang, Yak-Nam;Khokhlova, Tatiana;Bailey, Michael;Hwang, Joo Ha;Khokhlova, Vera
关键词：
最近的研究表明，高强度聚焦超声（HIFU）场中的冲击波加热和毫秒沸腾可导致组织的机械分离或乳化-称为沸腾组织摧毁术。颜色和含量变化的目视观察表明，乳化损伤中的热损伤程度可以通过改变暴露参数来控制。这项工作的目的是检查热和机械的影响，沸腾组织损伤，使用组织学和生化分析。使用2 MHz单元件传感器在离体牛心脏和肝脏中诱发损伤，工作占空比为0.005-0.01，脉冲持续时间为5-500 ms，原位冲击幅度为73 MPa。使用常规染色技术（H&E和NADH-双磷酸酶）在组织学上评估对组织的机械和热损伤。通过测量处理区域中盐溶性蛋白质的变性来量化热效应。根据组织学，病变在视觉上表现为液体，不含大于细胞核的细胞结构，并且具有1-2个细胞的非常尖锐的边界。组织学和蛋白质分析均显示，用短脉冲（< 10 ms）获得的损伤不包含任何热损伤。增加脉冲持续时间导致热损伤增加。然而，蛋白质分析和NADH-黄递酶染色显示变性比视觉观察到的组织变白更少。每次曝光的HIFU脉冲数并没有改变病变的形状或热变性的程度，而病变的大小显示出饱和的行为，从而表明最佳的曝光时间。这项研究证实，沸腾组织碎石术提供了一种有效的，可预测的方式，非侵入性骨折组织成亚细胞碎片，有或没有诱导热损伤。
Recent studies have shown that shock wave heating and millisecond boiling in high intensity focused ultrasound (HIFU) fields can result in mechanical fractionation or emulsification of tissue - named boiling histotripsy. Visual observations of the change in color and contents indicated that the degree of thermal damage in the emulsified lesions can be controlled by varying the parameters of the exposure. The goal of this work was to examine thermal and mechanical effects in boiling histotripsy lesions using histological and biochemical analysis. The lesions were induced in ex vivo bovine heart and liver using a 2-MHz single-element transducer operating at duty factors of 0.005–0.01, pulse durations of 5–500 ms, and in situ shock amplitude of 73 MPa. Mechanical and thermal damage to tissue was evaluated histologically using conventional staining techniques (H&E and NADH-diphorase). Thermal effects were quantified by measuring denaturation of salt soluble proteins in the treated region. According to histology, the lesions that visually appeared as a liquid, contained no cellular structures larger than a cell nucleus and had a very sharp border of 1–2 cells. Both histology and protein analysis showed that lesions obtained with short pulses (< 10 ms) did not contain any thermal damage. Increasing the pulse duration resulted in an increase in thermal damage. However, both protein analysis and NADH-diaphorase staining showed less denaturation than visually observed as whitening of tissue. The number of HIFU pulses delivered per exposure did not change the lesion shape or the degree of thermal denaturation, whereas the size of the lesion showed a saturating behaviour thus suggesting optimal exposure duration. This study confirmed that boiling histotripsy offers an effective, predictable way to non-invasively fractionate tissue into subcellular fragments with or without inducing thermal damage.
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DOI：
10.7863/jum.2012.31.4.623
发表时间：
2012-04
期刊：
Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine
影响因子：
--
作者：
Miller DL;Smith NB;Bailey MR;Czarnota GJ;Hynynen K;Makin IR;Bioeffects Committee of the American Institute of Ultrasound in Medicine
通讯作者：
Bioeffects Committee of the American Institute of Ultrasound in Medicine
影响因子：
2.4
作者：
Khokhlova, Tatiana D.;Canney, Michael S.;Bailey, Michael R.
通讯作者：
Bailey, Michael R.
影响因子：
--
作者：
Jung, Seung Eun;Cho, Se Hyun;Han, Joon-Yeol
通讯作者：
Han, Joon-Yeol
影响因子：
6.2
作者：
LAM, FT;MAVOR, AID;GILES, GR
通讯作者：
GILES, GR
影响因子：
2.9
作者：
Canney, Michael S.;Khokhlova, Vera A.;Bessonova, Olga V.;Bailey, Michael R.;Crum, Lawrence A.
通讯作者：
Crum, Lawrence A.