Yolk proteins of the schistosomiasis vector snail Biomphalaria glabrata revealed by multi-omics analysis.
Yolk proteins of the schistosomiasis vector snail Biomphalaria glabrata revealed by multi-omics analysis.
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DOI：
10.1038/s41598-024-52392-x
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发表时间：
2024-01-20
影响因子：
4.6
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中科院分区：
文献类型：
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卵黄发生是动物繁殖中最重要的过程，其中卵黄蛋白起着至关重要的作用。在多种卵黄蛋白前体中，卵黄蛋白原（Vtg）是大多数卵生动物中众所周知的主要卵黄蛋白（MYP）。然而，淡水腹足类蜗牛 Bimphalaria glabrata 中 MYP 的性质仍然难以捉摸。在当前的研究中，我们应用生物信息学、组织特异性转录组学、卵睾靶向蛋白质组学和系统发育学来研究光滑 B. glabrata 中的大脂质转移蛋白 (LLTP) 超家族和铁蛋白样家族。 在光滑双歧杆菌基因组中鉴定出 LLTP 超家族的四个成员（BgVtg1、BgVtg2、BgApo1 和 BgApo2）、一种卵黄铁蛋白（Bg yolk ferritin）和四种体体铁蛋白（Bg 铁蛋白 1、2、3 和 4）。蛋白质组分析表明，在假定的卵黄蛋白中，BgVtg1是卵睾中出现量最高的卵黄蛋白，其次是Bg卵黄铁蛋白。 RNAseq 分析显示，BgVtg1 和 Bg 卵黄铁蛋白的主要合成位点分别位于卵睾（可能是卵泡细胞）和消化腺。系统发育分析表明BgVtg1与其他脊椎动物和无脊椎动物的Vtgs很好地聚类。我们得出的结论是，卵黄蛋白原 (BgVtg1)，而不是卵黄铁蛋白 (Bg yolk ferritin)，是血吸虫病媒介蜗牛光滑 B. 的主要卵黄蛋白前体。
Vitellogenesis is the most important process in animal reproduction, in which yolk proteins play a vital role. Among multiple yolk protein precursors, vitellogenin (Vtg) is a well-known major yolk protein (MYP) in most oviparous animals. However, the nature of MYP in the freshwater gastropod snail Biomphalaria glabrata remains elusive. In the current study, we applied bioinformatics, tissue-specific transcriptomics, ovotestis-targeted proteomics, and phylogenetics to investigate the large lipid transfer protein (LLTP) superfamily and ferritin-like family in B. glabrata. Four members of LLTP superfamily (BgVtg1, BgVtg2, BgApo1, and BgApo2), one yolk ferritin (Bg yolk ferritin), and four soma ferritins (Bg ferritin 1, 2, 3, and 4) were identified in B. glabrata genome. The proteomic analysis demonstrated that, among the putative yolk proteins, BgVtg1 was the yolk protein appearing in the highest amount in the ovotestis, followed by Bg yolk ferritin. RNAseq profile showed that the leading synthesis sites of BgVtg1 and Bg yolk ferritin are in the ovotestis (presumably follicle cells) and digestive gland, respectively. Phylogenetic analysis indicated that BgVtg1 is well clustered with Vtgs of other vertebrates and invertebrates. We conclude that, vitellogenin (BgVtg1), not yolk ferritin (Bg yolk ferritin), is the major yolk protein precursor in the schistosomiasis vector snail B. glabrata.
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影响因子：
3.2
作者：
通讯作者：
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影响因子：
16.6
作者：
Adema CM;Hillier LW;Jones CS;Loker ES;Knight M;Minx P;Oliveira G;Raghavan N;Shedlock A;do Amaral LR;Arican-Goktas HD;Assis JG;Baba EH;Baron OL;Bayne CJ;Bickham-Wright U;Biggar KK;Blouin M;Bonning BC;Botka C;Bridger JM;Buckley KM;Buddenborg SK;Lima Caldeira R;Carleton J;Carvalho OS;Castillo MG;Chalmers IW;Christensens M;Clifton S;Cosseau C;Coustau C;Cripps RM;Cuesta-Astroz Y;Cummins SF;di Stephano L;Dinguirard N;Duval D;Emrich S;Feschotte C;Feyereisen R;FitzGerald P;Fronick C;Fulton L;Galinier R;Gava SG;Geusz M;Geyer KK;Giraldo-Calderón GI;de Souza Gomes M;Gordy MA;Gourbal B;Grunau C;Hanington PC;Hoffmann KF;Hughes D;Humphries J;Jackson DJ;Jannotti-Passos LK;de Jesus Jeremias W;Jobling S;Kamel B;Kapusta A;Kaur S;Koene JM;Kohn AB;Lawson D;Lawton SP;Liang D;Limpanont Y;Liu S;Lockyer AE;Lovato TL;Ludolf F;Magrini V;McManus DP;Medina M;Misra M;Mitta G;Mkoji GM;Montague MJ;Montelongo C;Moroz LL;Munoz-Torres MC;Niazi U;Noble LR;Oliveira FS;Pais FS;Papenfuss AT;Peace R;Pena JJ;Pila EA;Quelais T;Raney BJ;Rast JP;Rollinson D;Rosse IC;Rotgans B;Routledge EJ;Ryan KM;Scholte LLS;Storey KB;Swain M;Tennessen JA;Tomlinson C;Trujillo DL;Volpi EV;Walker AJ;Wang T;Wannaporn I;Warren WC;Wu XJ;Yoshino TP;Yusuf M;Zhang SM;Zhao M;Wilson RK
通讯作者：
Wilson RK
影响因子：
3.6
作者：
Brooks, JM;Wessel, GM
通讯作者：
Wessel, GM
影响因子：
3.6
作者：
DEJONGBRINK, M;DEWIT, A;BOER, HH
通讯作者：
BOER, HH
影响因子：
3.4
作者：
Avarre JC;Lubzens E;Babin PJ
通讯作者：
Babin PJ