Apolipocrustacein, formerly vitellogenin, is the major egg yolk precursor protein in decapod crustaceans and is homologous to insect apolipophorin II/I and vertebrate apolipoprotein B.
Apolipocrustacein, formerly vitellogenin, is the major egg yolk precursor protein in decapod crustaceans and is homologous to insect apolipophorin II/I and vertebrate apolipoprotein B.
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DOI：
10.1186/1471-2148-7-3
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发表时间：
2007-01-22
影响因子：
3.4
通讯作者：
Babin PJ
中科院分区：
文献类型：
--
作者：
Avarre JC;Lubzens E;Babin PJ
在动物中，某些脂蛋白类的生物发生需要古老的大脂质转移蛋白（LLTP）超家族的成员，包括微粒体甘油三酯转移蛋白（MTP）、脊椎动物载脂蛋白B（apo B）、卵黄蛋白原（Vtg）和昆虫载脂蛋白II/I前体（apoLp-II/I）的胞质大亚基。在大多数卵生物种中，Vtg是一种大的糖脂蛋白，是主要的蛋黄前体蛋白。本报告阐明了LLTP超家族成员的系统发育关系，并将其分为三个科及其相关的亚科。这意味着通用术语Vtg不再是功能术语，而是基于系统发育/结构标准。此外，我们确定的主要卵黄前体蛋白的十足类甲壳动物显示出更大的整体序列相似性与apoLp-II/I比其他LLTP，包括Vtgs。这种密切的关联得到了保守序列基序的系统发育分析（即邻居连接、最大似然和贝叶斯推理方法）以及三个常见保守结构域的存在的支持：LLTP的N-末端大脂质转移模块标记物，在其中心区域中与apoLp-II/I和apoB专有共享的未知功能的DUF 1081结构域，和在其C-末端的冯维勒布兰德因子D型结构域。此外，它们与apoLp-II/I在相似的位置共享保守的功能性枯草杆菌蛋白酶样内切蛋白酶切割位点。的结构和系统发育数据表明，十足类甲壳动物的主要蛋黄前体蛋白是令人惊讶的密切相关的昆虫载脂蛋白-II/I和脊椎动物载脂蛋白B，应该被称为载脂甲壳素（apoCr），而不是VTG。这些LLTP可能来自古老的重复事件，导致Vtg序列的旁系同源物。LLTP同源物在一个基因组中的存在可以促进冗余，例如参与脂质代谢和作为蛋黄前体蛋白，以及细胞外LLTP成员的新功能化和亚功能化，例如参与凝血级联和免疫应答。这些编码蛋白质的核基因可用于解决主要节肢动物类群之间的系统发育关系，特别是Pancrustacea主要分裂。
In animals, the biogenesis of some lipoprotein classes requires members of the ancient large lipid transfer protein (LLTP) superfamily, including the cytosolic large subunit of microsomal triglyceride transfer protein (MTP), vertebrate apolipoprotein B (apoB), vitellogenin (Vtg), and insect apolipophorin II/I precursor (apoLp-II/I). In most oviparous species, Vtg, a large glycolipoprotein, is the main egg yolk precursor protein. This report clarifies the phylogenetic relationships of LLTP superfamily members and classifies them into three families and their related subfamilies. This means that the generic term Vtg is no longer a functional term, but is rather based on phylogenetic/structural criteria. In addition, we determined that the main egg yolk precursor protein of decapod crustaceans show an overall greater sequence similarity with apoLp-II/I than other LLTP, including Vtgs. This close association is supported by the phylogenetic analysis, i.e. neighbor-joining, maximum likelihood and Bayesian inference methods, of conserved sequence motifs and the presence of three common conserved domains: an N-terminal large lipid transfer module marker for LLTP, a DUF1081 domain of unknown function in their central region exclusively shared with apoLp-II/I and apoB, and a von Willebrand-factor type D domain at their C-terminal end. Additionally, they share a conserved functional subtilisin-like endoprotease cleavage site with apoLp-II/I, in a similar location. The structural and phylogenetic data presented indicate that the major egg yolk precursor protein of decapod crustaceans is surprisingly closely related to insect apoLp-II/I and vertebrate apoB and should be known as apolipocrustacein (apoCr) rather than Vtg. These LLTP may arise from an ancient duplication event leading to paralogs of Vtg sequences. The presence of LLTP homologs in one genome may facilitate redundancy, e.g. involvement in lipid metabolism and as egg yolk precursor protein, and neofunctionalization and subfunctionalization, e.g. involvement in clotting cascade and immune response, of extracellular LLTP members. These protein-coding nuclear genes may be used to resolve phylogenetic relationships among the major arthropod groups, especially the Pancrustacea-major splits.
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DOI：
10.1073/pnas.96.5.1965
发表时间：
1999-03-02
影响因子：
11.1
作者：
Hall, M;Wang, RG;Söderhäll, K
通讯作者：
Söderhäll, K
影响因子：
3.9
作者：
Chen, JS;Sappington, TW;Raikhel, AS
通讯作者：
Raikhel, AS
影响因子：
2.7
作者：
Abdu, U;Davis, C;Sagi, A
通讯作者：
Sagi, A
影响因子：
3.5
作者：
Kato, Y;Tokishita, S;Yamagata, H
通讯作者：
Yamagata, H
影响因子：
11.4
作者：
BUJO, H;HERMANN, M;SCHNEIDER, WJ
通讯作者：
SCHNEIDER, WJ