Chronic lead exposure reduces doublecortin-expressing immature neurons in young adult guinea pig cerebral cortex.
Chronic lead exposure reduces doublecortin-expressing immature neurons in young adult guinea pig cerebral cortex.
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慢性铅暴露会减少年轻成年豚鼠大脑皮层中表达双皮质素的未成熟神经元
DOI：
10.1186/1471-2202-13-82
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发表时间：
2012-07-19
期刊：
影响因子：
2.4
通讯作者：
Xiong K
中科院分区：
文献类型：
--
作者：
Huang J;Huang K;Shang L;Wang H;Zhang M;Fan CL;Chen D;Yan X;Xiong K
慢性铅（Pb）中毒仍然是一种环境风险，特别是对儿童人群，它可能会影响大脑发育。表达双皮质素（DCX+）的未成熟神经元存在于各种哺乳动物的皮质层II周围，包括成年豚鼠和人类。本研究以年轻成年豚鼠为实验模型，探讨慢性铅暴露是否影响皮层DCX +未成熟神经元和室下区和颗粒下区（SVZ，SGZ）周围的神经元。用含0.2%醋酸铅的饮用水对两个月大的豚鼠进行2、4和6个月的处理。在上述时间点，这些动物的血铅水平分别达到10.27 ± 0.62、16.25 ± 0.78和19.03 ± 0.86 μg/dL，而溶剂对照组的血铅水平约为3 μg/dL。DCX +神经元的密度显着降低周围皮质层II，SVZ和SGZ在铅治疗的动物存活4个月和6个月相对于对照组。溴脱氧尿苷（BrdU）脉冲追踪研究未能找到细胞共定位的DNA合成指示剂在DCX +细胞周围的第二层铅处理和对照动物。未发现这些皮质未成熟神经元与活性caspase-3或Fluoro-Jade C标记共存。慢性铅暴露可导致年轻成年豚鼠皮层第二层周围和常规神经原性部位的未成熟神经元数量显著减少。没有直接的证据表明皮质DCX表达的减少与局部神经发生或神经元死亡的改变有关。
Chronic lead (Pb) poisoning remains an environmental risk especially for the pediatric population, and it may affect brain development. Immature neurons expressing doublecortin (DCX+) exist around cortical layer II in various mammals, including adult guinea pigs and humans. Using young adult guinea pigs as an experimental model, the present study explored if chronic Pb exposure affects cortical DCX + immature neurons and those around the subventricular and subgranular zones (SVZ, SGZ). Two month-old guinea pigs were treated with 0.2% lead acetate in drinking water for 2, 4 and 6 months. Blood Pb levels in these animals reached 10.27 ± 0.62, 16.25 ± 0.78 and 19.03 ± 0.86 μg/dL at the above time points, respectively, relative to ~3 μg/dL in vehicle controls. The density of DCX + neurons was significantly reduced around cortical layer II, SVZ and SGZ in Pb-treated animals surviving 4 and 6 months relative to controls. Bromodeoxyuridine (BrdU) pulse-chasing studies failed to find cellular colocalization of this DNA synthesis indicator in DCX + cells around layer II in Pb-treated and control animals. These cortical immature neurons were not found to coexist with active caspase-3 or Fluoro-Jade C labeling. Chronic Pb exposure can lead to significant reduction in the number of the immature neurons around cortical layer II and in the conventional neurogenic sites in young adult guinea pigs. No direct evidence could be identified to link the reduced cortical DCX expression with alteration in local neurogenesis or neuronal death.
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影响因子：
4.9
作者：
Bleecker, ML;Ford, DP;Bleecker, ML
通讯作者：
Bleecker, ML
DOI：
10.1016/j.ijdevneu.2005.07.005
发表时间：
2005-11-01
影响因子：
1.8
作者：
Jaako-Movits, K;Zharkovsky, T;Zharkovsky, A
通讯作者：
Zharkovsky, A
影响因子：
5.3
作者：
Cai, Yan;Xiong, Kun;Chu, Yaping;Luo, Duan-Wu;Luo, Xue-Gang;Yuan, Xian-Yui;Struble, Robert G.;Clough, Richard W.;Spencer, Dennis D.;Williamson, Anne;Kordower, Jeffrey H.;Patrylo, Peter R.;Yan, Xiao-Xin
通讯作者：
Yan, Xiao-Xin
影响因子：
3.7
作者：
Klempin F;Kronenberg G;Cheung G;Kettenmann H;Kempermann G
通讯作者：
Kempermann G
影响因子：
158.5
作者：
Canfield, RL;Henderson, CR;Lanphear, BP
通讯作者：
Lanphear, BP