Doublecortin expression in adult cat and primate cerebral cortex relates to immature neurons that develop into GABAergic subgroups.
Doublecortin expression in adult cat and primate cerebral cortex relates to immature neurons that develop into GABAergic subgroups.
复制标题
DOI：
10.1016/j.expneurol.2008.12.008
复制
发表时间：
2009-04
影响因子：
5.3
通讯作者：
Yan, Xiao-Xin
中科院分区：
文献类型：
--
作者：
Cai, Yan;Xiong, Kun;Chu, Yaping;Luo, Duan-Wu;Luo, Xue-Gang;Yuan, Xian-Yui;Struble, Robert G.;Clough, Richard W.;Spencer, Dennis D.;Williamson, Anne;Kordower, Jeffrey H.;Patrylo, Peter R.;Yan, Xiao-Xin
DCX免疫反应（DCX+）细胞出现在成年小鼠和大鼠的梨状皮质中，但也出现在成年豚鼠和兔的新皮质中。在这里，我们描述了成年家猫和灵长类动物的这些细胞。在猫和恒河猴中，DCX+细胞存在于异位和新皮质中，在给定的额平面上整体呈腹背高到低梯度。标记的细胞在第二层和第三层上部形成细胞带，在染色体大小（5-20 μm）、形状（单极、双极、多极和不规则）、神经鞘复杂性和标记强度方面存在显著差异。在这一波段也可见到细胞簇，并且内嗅皮层的细胞簇以链状结构向更深层延伸。密度测量显示，随着年龄的增长，猫的各个区域的细胞也在平行下降。除细胞带外，DCX反应性较弱的中等细胞稀疏分布于其他层。在整个皮层中，几乎所有DCX+细胞都共表达了多唾液化的神经细胞粘附分子。中大型成熟型DCX+细胞经常与神经元特异性核蛋白和γ-氨基丁酸（GABA）共定位，而DCX表达降低的细胞也部分共标记谷氨酸脱羧酶、小白蛋白、钙结合蛋白、β-烟酰胺腺嘌呤二核苷酸磷酸二磷酸和神经元一氧化氮合酶。与猫和猴子类似，在手术切除的人类额叶和颞叶皮层中检测到大小不同的DCX+细胞。这些数据表明，在猫和灵长类动物的许多皮质区域中，未成熟的神经元持续存在到成年，并且这些细胞似乎经历了发育和分化，成为gaba能中间神经元的功能亚群。
DCX-immunoreactive (DCX+) cells occur in the piriform cortex in adult mice and rats, but also in the neocortex in adult guinea pigs and rabbits. Here we describe these cells in adult domestic cats and primates. In cats and rhesus monkeys, DCX+ cells existed across the allo- and neocortex, with an overall ventrodorsal high to low gradient at a given frontal plane. Labeled cells formed a cellular band in layers II and upper III, exhibiting dramatic differences in somal size (5–20 μm), shape (unipolar, bipolar, multipolar and irregular), neuritic complexity and labeling intensity. Cell clusters were also seen in this band, and those in the entorhinal cortex extended into deeper layers as chain-like structures. Densitometry revealed a parallel decline of the cells across regions with age in cats. Besides the cellular band, medium-sized cells with weak DCX reactivity resided sparsely in other layers. Throughout the cortex, virtually all DCX+ cells co-expressed polysialylated neural cell adhesion molecule. Medium to large mature-looking DCX+ cells frequently colocalized with neuron-specific nuclear protein and γ-aminobutyric acid (GABA), and those with a reduced DCX expression also partially co-labeled for glutamic acid decarboxylase, parvalbumin, calbindin, β-nicotinamide adenine dinucleotide phosphate diaphorase and neuronal nitric oxide synthase. Similar to cats and monkeys, small and larger DCX+ cells were detected in surgically removed human frontal and temporal cortices. These data suggest that immature neurons persist into adulthood in many cortical areas in cats and primates, and that these cells appear to undergo development and differentiation to become functional subgroups of GABAergic interneurons.
登录
查看更多内容
影响因子：
2.5
作者：
Brown, JP;Couillard-Després, S;Kuhn, HG
通讯作者：
Kuhn, HG
影响因子：
3.4
作者：
Couillard-Despres, S;Winner, B;Aigner, L
通讯作者：
Aigner, L
影响因子：
2.5
作者：
HENDRICKSON, AE;VANBREDERODE, JFM;CELIO, MR
通讯作者：
CELIO, MR
影响因子：
2.5
作者：
ALCANTARA, S;FERRER, I
通讯作者：
FERRER, I
影响因子：
6.1
作者：
Chu, Yaping;Kordower, Jeffrey H.
通讯作者：
Kordower, Jeffrey H.