Coupling Seq-BSA and RNA-Seq Analyses Reveal the Molecular Pathway and Genes Associated with Heading Type in Chinese Cabbage.
Coupling Seq-BSA and RNA-Seq Analyses Reveal the Molecular Pathway and Genes Associated with Heading Type in Chinese Cabbage.
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Seq-BSA 和 RNA-Seq 耦合分析揭示了大白菜抽穗类型相关的分子途径和基因
DOI：
10.3389/fgene.2017.00176
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发表时间：
2017
影响因子：
3.7
通讯作者：
Shen S
中科院分区：
文献类型：
--
作者：
Gu A;Meng C;Chen Y;Wei L;Dong H;Lu Y;Wang Y;Chen X;Zhao J;Shen S
结球型是大白菜的一个重要农艺性状，具有重要的经济意义。使用天然小孢子衍生的双单倍体植物，我们产生了具有重叠或向外卷曲头部形态型的自交后代。基于测序的混合分离子分析（Seq-BSA）显示了一个0.52 Mb（A06：1，824，886 ~ 2，347，097 bp）的候选区域，该区域含有植物激素信号转导富集基因。RNA测序（RNA-Seq）分析支持激素途径富集，导致两个关键候选基因BrGH3.12和BrABF 1的鉴定。揭示了该途径中受调控的同源基因及基因间的相互关系。BrGH3.12的表达在叶的顶端部分变化显著，与重叠和向外卷曲的叶之间的形态差异一致。BrABF 1在叶片上、中、基部的转录水平在两种类型之间存在显著差异。这两种形态型含有不同浓度的IAA在其叶的顶端部分，而阿坝的水平在顶部，中部和基部叶段的植物类型之间的差异显着。来自Seq-BSA、RNA-Seq和代谢物分析的结果都支持IAA和阿坝在抽穗类型形成中的作用。这些发现增加了我们对大白菜叶球形态形成的分子基础的理解，并将有助于未来开发更理想的叶球形态。
In Chinese cabbage, heading type is a key agricultural trait of significant economic importance. Using a natural microspore-derived doubled haploid plant, we generated self-crossed progeny with overlapping or outward curling head morphotypes. Sequencing-based bulked segregant analysis (Seq-BSA) revealed a candidate region of 0.52 Mb (A06: 1,824,886~2,347,097 bp) containing genes enriched for plant hormone signal transduction. RNA Sequencing (RNA-Seq) analysis supported the hormone pathway enrichment leading to the identification of two key candidate genes, BrGH3.12 and BrABF1. The regulated homologous genes and the relationship between genes in this pathway were also revealed. Expression of BrGH3.12 varied significantly in the apical portion of the leaf, consistent with the morphological differences between overlapping and outward curling leaves. Transcript levels of BrABF1 in the top, middle and basal segments of the leaf were significantly different between the two types. The two morphotypes contained different concentrations of IAA in the apical portion of their leaves while levels of ABA differed significantly between plant types in the top, middle, and basal leaf segments. Results from Seq-BSA, RNA-Seq and metabolite analyses all support a role for IAA and ABA in heading type formation. These findings increase our understanding of the molecular basis for pattern formation of the leafy head in Chinese cabbage and will contribute to future work developing more desirable leafy head patterns.
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影响因子：
10.5
作者：
Cheng, Youfa;Dai, Xinhua;Zhao, Yunde
通讯作者：
Zhao, Yunde
影响因子：
5.3
作者：
HAGEN, G;GUILFOYLE, TJ
通讯作者：
GUILFOYLE, TJ
影响因子：
11.6
作者：
Braun, Nils;Wyrzykowska, Joanna;Fleming, Andrew J.
通讯作者：
Fleming, Andrew J.
影响因子：
2.9
作者：
Bitto, Eduard;Bingman, Craig A.;Bittova, Lenka;Houston, Norma L.;Boston, Rebecca S.;Fox, Brian G.;Phillips, George N., Jr.
通讯作者：
Phillips, George N., Jr.
影响因子：
2.4
作者：
Gu AX;Zhao JJ;Li LM;Wang YH;Zhao YJ;Hua F;Xu YC;Shen SX
通讯作者：
Shen SX