Live and let die - the B(sister) MADS-box gene OsMADS29 controls the degeneration of cells in maternal tissues during seed development of rice (Oryza sativa).
Live and let die - the B(sister) MADS-box gene OsMADS29 controls the degeneration of cells in maternal tissues during seed development of rice (Oryza sativa).
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生而死——Bsister MADS-Box 基因 OsMADS29 控制水稻 (Oryza sativa) 种子发育过程中母体组织细胞的退化
DOI：
10.1371/journal.pone.0051435
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Meng Z
中科院分区：
文献类型：
--
作者：
Yang X;Wu F;Lin X;Du X;Chong K;Gramzow L;Schilling S;Becker A;Theißen G;Meng Z
Bsister基因被认为是B类花同源异型基因的近亲。以往的研究表明，真双子叶植物的Bsister基因参与胚珠和种子发育过程中的细胞分化。然而，Bsister基因在真双子叶植物中的完整功能被与其他基因的冗余所掩盖，并且对Bsister基因在单子叶植物中的功能以及Bsister基因功能的进化知之甚少。在这里，我们描述了水稻中三个MADS盒B姐妹基因之一OsMADS 29的特征。我们的分析表明，OsMADS 29在雌性生殖器官中表达，包括胚珠、胚珠脉管系统和整个种子，除了果皮的外层细胞。通过双链RNA介导的干扰（RNAi）敲低OsMADS 29导致种子皱缩和/或败育。授粉后7天对异常种子的组织学分析表明，共质体的连续性，包括胚珠维管痕迹和珠心突起，这是子代组织在早期发育阶段的营养来源，受到影响。此外，与对照植物相比，转基因种子中的所有母体组织（包括果皮、胚珠维管痕迹、珠被、珠心表皮和珠心突起）的退化被阻断。这些结果表明OsMADS 29基因通过调控母体组织的细胞退化在水稻种子发育中发挥重要作用。我们的研究结果为Bsister基因的祖先功能提供了重要的见解。
Bsister genes have been identified as the closest relatives of class B floral homeotic genes. Previous studies have shown that Bsister genes from eudicots are involved in cell differentiation during ovule and seed development. However, the complete function of Bsister genes in eudicots is masked by redundancy with other genes and little is known about the function of Bsister genes in monocots, and about the evolution of Bsister gene functions. Here we characterize OsMADS29, one of three MADS-box Bsister genes in rice. Our analyses show that OsMADS29 is expressed in female reproductive organs including the ovule, ovule vasculature, and the whole seed except for the outer layer cells of the pericarp. Knock-down of OsMADS29 by double-stranded RNA-mediated interference (RNAi) results in shriveled and/or aborted seeds. Histological analyses of the abnormal seeds at 7 days after pollination (DAP) indicate that the symplastic continuity, including the ovular vascular trace and the nucellar projection, which is the nutrient source for the filial tissue at early development stages, is affected. Moreover, degeneration of all the maternal tissues in the transgenic seeds, including the pericarp, ovular vascular trace, integuments, nucellar epidermis and nucellar projection, is blocked as compared to control plants. Our results suggest that OsMADS29 has important functions in seed development of rice by regulating cell degeneration of maternal tissues. Our findings provide important insights into the ancestral function of Bsister genes.
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大米中的MADS-box基因家族：在生殖发育和压力期间，全基因组识别，组织和表达分析。
影响因子：
4.4
作者：
Arora, Rita;Agarwal, Pinky;Ray, Swatismita;Singh, Ashok Kumar;Singh, Vijay Pal;Tyagi, Akhilesh K.;Kapoor, Sanjay
通讯作者：
Kapoor, Sanjay
DOI：
10.1016/s0167-4889(03)00102-2
发表时间：
2003-09-23
影响因子：
5.1
作者：
Godbole, A;Varghese, J;Mathew, MK
通讯作者：
Mathew, MK
影响因子：
10.7
作者：
Becker, A;Winter, KU;Theissen, G
通讯作者：
Theissen, G
影响因子：
7.2
作者：
Erdmann, Robert;Gramzow, Lydia;Becker, Annette
通讯作者：
Becker, Annette
DOI：
10.1006/bbrc.2001.5507
发表时间：
2001-09-07
影响因子：
3.1
作者：
Jwa, NS;Agrawal, GK;Agrawal, VP
通讯作者：
Agrawal, VP