Cleavage of hepatitis B virus RNA by three ribozymes transcribed from a single DNA template.
Cleavage of hepatitis B virus RNA by three ribozymes transcribed from a single DNA template.
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由单一 DNA 模板转录的三种核酶裂解乙型肝炎病毒 RNA。
DOI：
10.1016/0006-291x(92)92264-x
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发表时间：
1992
影响因子：
3.1
通讯作者：
Wands,JR
中科院分区：
文献类型：
--
作者：
vonWeizsäcker,F;Blum,HE;Wands,JR
B型肝炎病毒是一种DNA病毒，通过RNA中间体（前基因组RNA）的逆转录进行不对称复制。作为抗病毒策略的第一步，在体外合成、克隆和转录编码针对前基因组RNA内三个相邻位点的3个核酶基序的单个合成寡脱氧核苷酸。利用RNA的5′和3′末端标记的实验表明，三种核酶准确地切割B型肝炎病毒底物RNA。切割效率与单核酶构建体的切割效率相似。这些结果表明，B型肝炎病毒RNA对核酶诱导的切割敏感，并说明当从单个DNA模板转录时，三种核酶同时具有活性。如果存在高病毒靶序列变异性，则多个核酶基序的表达可提供优势。
Hepatitis B virus is a DNA virus which replicates asymmetrically through reverse transcription of an RNA intermediate (pregenomic RNA). As a first step toward an antiviral strategy, a single synthetic oligodeoxynucleotide coding for 3 ribozyme motifs directed against three adjacent sites within the pregenomic RNA was synthesized, cloned and transcribed in vitro. Experiments utilizing 5′ and 3′ end labeling of RNA demonstrated that the three ribozymes accurately cleaved hepatitis B virus substrate RNA. Cleavage efficiency was similar to that of single ribozyme constructs. These results demonstrate that hepatitis B virus RNA is susceptible to ribozyme induced cleavage and illustrate that three ribozymes were simultaneously active when transcribed from a single DNA template. The expression of multiple ribozyme motifs may offer an advantage if there is high viral target sequence variability.
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影响因子：
2.9
作者：
RUFFNER, DE;STORMO, GD;UHLENBECK, OC
通讯作者：
UHLENBECK, OC
影响因子：
14.9
作者：
CHEN, CJ;BANERJEA, AC;SCHUBERT, M
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SCHUBERT, M
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56.9
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ROSSI, JJ
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VARMUS, HE
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56.9
作者：
CECH, TR
通讯作者：
CECH, TR