Effects of toxic Microcystis aeruginosa on the expression of Hox genes in Daphnia similoides sinensis.
Effects of toxic Microcystis aeruginosa on the expression of Hox genes in Daphnia similoides sinensis.
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有毒铜绿微囊藻对中华溞Hox基因表达的影响
DOI：
10.1002/ece3.8685
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发表时间：
2022-03
影响因子：
2.6
通讯作者：
Zhou Z
中科院分区：
文献类型：
--
作者：
Xu X;Cao Y;Qi H;Deng D;Zhang YN;Wu J;Peng S;Zhou Z
湖泊富营养化和蓝藻水华已成为世界性的环境问题。在蓝藻水华（特别是微囊藻）下，水蚤属。可以将有益的信息传递给后代，以提高适应能力。Hox基因是后生动物中重要的转录调控因子，参与生物体的生长发育。然而，微囊藻对水蚤Hox基因表达的影响机制尚不清楚。本研究采用铜绿微囊藻（Microcystis aeruginosa）与相似水蚤（Daphnia similoides sinensis）的混合饵料，研究了铜绿微囊藻对相似水蚤（Daphnia similoides sinensis）两个无性系母体和子代（F1）Hox基因表达的影响。铜绿假单胞菌（Scenedesmus aeruginosa）和栅藻（Scenedesmus aeruginosa）。与100%S食物处理相比，克隆1-F1在含有M.在20%M + 80%S食物处理中，对于铜绿假单胞菌，其显著更低，但对于克隆2-F1，其显著更高。此外，克隆1-F1在含有M.的食物处理中实验结束时的存活率。铜绿假单胞菌的含量显著高于母亲。根据已有的转录组数据，对D.鉴定出的相似拟步行虫包括Abd-B、CDX-1、Dll、HOX-1、HOX-2、HOXA 1、HOXA 2、HOXB 3、HOXB 3 - 2、HOXB 7、HOXC 4、HOXC 7、HOXC 8和HOXD 10。在40%M + 60%S食物处理中，克隆2母亲的Abd-B、HOX-2、HOXA 1、HOXC 7和HOXD 10的表达是100%S食物处理中的2.9-22.5倍，而克隆1母亲的CDX-1、HOX-1、HOXB 3和HOXD 10的表达在相同食物水平下是4.8-13.1倍。在40%M + 60%S食物处理中，克隆I-F1的HOXA 2、HOXC 7、HOXC 8和HOXD 10的表达是100%S食物处理中的8.2-21.1倍。然而，与100%S食物处理相比，在含有M.铜绿假单胞菌显著降低（P <0.05）。结果表明，D.中华拟步行虫母体预先暴露于有毒的M.铜绿假单胞菌对M.铜绿比他们的母亲。Hox基因在D.在有毒M.铜绿。D.不同处理下的中华绒螯蟹蓝藻可能通过母体效应影响水蚤Hox基因的表达。Hox基因在D.在有毒M.铜绿。
Lake eutrophication and cyanobacterial blooms have become worldwide environmental issues. Under cyanobacterial blooms (especially Microcystis), Daphnia spp. can transfer beneficial information to their offspring in order to improve adaptability. Hox genes are important regulatory factors of transcription in metazoans, and are involved in the growth and development of organisms. However, the mechanisms of Microcystis on the expression of Hox genes in Daphnia are unclear. In this study, the effects of Microcystis aeruginosa on Hox gene expression in the mothers and offspring (F1) of two Daphnia similoides sinensis clones were investigated using a mixed diet of M. aeruginosa and Scenedesmus obliquus. Compared with the 100%S food treatment, the survival rates at the end of the experiment of clone 1‐F1 in the food treatments containing M. aeruginosa were significantly lower, but it was significantly higher for clone 2‐F1 in the 20%M + 80%S food treatment. Moreover, the survival rates at the end of the experiment of clone 1‐F1 in the food treatments containing M. aeruginosa were significantly higher than those of their mother. Based on previous transcriptome data, 14 Hox genes of D. similoides sinensis were identified, including Abd‐B, CDX‐1, Dll, HOX‐1, HOX‐2, HOXA1, HOXA2, HOXB3, HOXB3‐2, HOXB7, HOXC4, HOXC7, HOXC8, and HOXD10. The expressions of Abd‐B, HOX‐2, HOXA1, HOXC7, and HOXD10 of clone 2‐mothers in the 40%M + 60%S food treatment were 2.9–22.5 times as high as in the 100%S food treatment, whereas the expressions of CDX‐1, HOX‐1, HOXB3, and HOXD10 of clone 1‐mothers were 4.8–13.1 times at same food level. The expression of HOXA2, HOXC7, HOXC8, and HOXD10 of clone 1‐F1 in the 40%M + 60%S food treatment was 8.2–21.1 times as high as in the 100%S food treatment. However, compared with the 100%S food treatment, the expressions of CDX‐1 in the mothers and F1 of clone 2 and HOXB7 in the mothers of clone 1 in the food treatments containing M. aeruginosa were significantly lower (p < .05). Our results suggest that the offspring (F1) produced by D. similoides sinensis mother pre‐exposed to toxic M. aeruginosa had stronger adaptability to M. aeruginosa than their mothers. Moreover, Hox gene expressions of D. similoides sinensis had obvious differences between clones under stress of toxic M. aeruginosa. There were significant differences in the survival rates of D. similoides sinensis under different treatments. Cyanobacteria may be able to affect the expression of Hox genes in Daphnia via maternal effects. Hox gene expressions of D. similoides sinensis had obvious differences between clones under stress of toxic M. aeruginosa.
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影响因子：
2.7
作者：
Frost, Paul C.;Ebert, Dieter;Zalewski, Alexandra
通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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