RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate.
RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate.
复制标题
作者：
病毒RNA可被Toll样受体7和8或RNA解旋酶LGP2、MDA5和RIG - I识别，从而触发抗病毒反应。对于DNA的识别受体人们所知甚少。在此我们确定了一种涉及RNA聚合酶III和RIG - I的新型DNA识别通路。富含AT的双链DNA作为RNA聚合酶III的模板，被转录为带有5′三磷酸部分的双链RNA，它通过RIG - I发出信号，激活I型干扰素基因转录和核因子κB。这一通路在识别爱泼斯坦 - 巴尔病毒编码的小RNA时也很重要，这些小RNA由RNA聚合酶III转录，然后触发RIG - I的激活。因此，RNA聚合酶III和RIG - I在先天免疫应答中协调抗病毒防御方面起着关键作用。
Viral RNA is sensed by TLR 7 and 8 or by the RNA helicases LGP2, MDA5 and RIG-I to trigger antiviral responses. Much less is known about sensors for DNA. Here we identify a novel DNA sensing pathway involving RNA polymerase III and RIG-I. AT-rich dsDNA serve as a template for RNA polymerase III, which is transcribed into dsRNA harboring a 5′ triphosphate moiety which signals via RIG-I to activate type I IFN gene transcription and NF-κB. This pathway is also important in sensing Epstein-Barr virus encoded small RNAs, which are transcribed by RNA polymerase III and then trigger RIG-I activation. Thus, RNA Pol III and RIG-I play a pivotal role in coordinating anti-viral defenses in the innate immune response.
登录
查看更多内容
影响因子：
56.9
作者：
Pichlmair, Andreas;Schulz, Oliver;Sousa, Caetano Reis E.
通讯作者：
Sousa, Caetano Reis E.
影响因子：
30.5
作者：
Buerckstuemmer, Tilmann;Baumann, Christoph;Superti-Furga, Giulio
通讯作者：
Superti-Furga, Giulio
影响因子：
64.8
作者：
Meylan, E;Curran, J;Tschopp, R
通讯作者：
Tschopp, R
DOI：
10.1073/pnas.78.2.805
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LERNER, MR;ANDREWS, NC;STEITZ, JA
通讯作者：
STEITZ, JA
影响因子：
30.5
作者：
Kawai, T;Takahashi, K;Akira, S
通讯作者：
Akira, S