MicroRNA Expression Profile of Neural Progenitor-Like Cells Derived from Rat Bone Marrow Mesenchymal Stem Cells under the Influence of IGF-1, bFGF and EGF.
MicroRNA Expression Profile of Neural Progenitor-Like Cells Derived from Rat Bone Marrow Mesenchymal Stem Cells under the Influence of IGF-1, bFGF and EGF.
复制标题
DOI：
10.3390/ijms16059693
复制
发表时间：
2015-04-29
影响因子：
5.6
通讯作者：
Jaafar H
中科院分区：
文献类型：
--
作者：
Huat TJ;Khan AA;Abdullah JM;Idris FM;Jaafar H
胰岛素样生长因子1（IGF-1）在表皮生长因子（EGF）和碱性成纤维细胞生长因子（bFGF）存在下促进骨髓间充质干细胞（BMSCs）向神经祖细胞样细胞（NPC）分化的早期过程中促进细胞增殖并减少凋亡。BMSCs在三组生长因子中分化：（A）EGF + bFGF，（B）EGF + bFGF + IGF-1，和（C）不含生长因子。为了阐明NPC衍生的分子机制，使用GeneChip® miRNA阵列进行微阵列分析。比较各组间的特征。注释的microRNA指纹（GSE 60060）描绘了与C组相比暂时上调或下调的46个microRNA。通过实时PCR验证所选microRNA的表达。在46种microRNA中，30种在至少两个连续时间间隔内一致表达。在B组中，只有miR-496上调，12种microRNA下调，包括let-7家族、miR-1224、miR-125 a-3 p、miR-214、miR-22、miR-320、miR-708和miR-93。生物信息学分析显示，这些microRNA（miR-22、miR-214、miR-125 a-3 p、miR-320和let-7家族）中的一些与细胞凋亡的减少相关。在此，我们总结了与IGF-1相关的关键microRNA在BMSCs向NPC分化中的作用。这些发现可能为我们进一步理解microRNA作为BMSC来源的NPC维持的关键调节因子的机制和作用提供线索。
Insulin-like growth factor 1 (IGF-1) enhances cellular proliferation and reduces apoptosis during the early differentiation of bone marrow derived mesenchymal stem cells (BMSCs) into neural progenitor-like cells (NPCs) in the presence of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). BMSCs were differentiated in three groups of growth factors: (A) EGF + bFGF, (B) EGF + bFGF + IGF-1, and (C) without growth factor. To unravel the molecular mechanisms of the NPCs derivation, microarray analysis using GeneChip® miRNA arrays was performed. The profiles were compared among the groups. Annotated microRNA fingerprints (GSE60060) delineated 46 microRNAs temporally up-regulated or down-regulated compared to group C. The expressions of selected microRNAs were validated by real-time PCR. Among the 46 microRNAs, 30 were consistently expressed for minimum of two consecutive time intervals. In Group B, only miR-496 was up-regulated and 12 microRNAs, including the let-7 family, miR-1224, miR-125a-3p, miR-214, miR-22, miR-320, miR-708, and miR-93, were down-regulated. Bioinformatics analysis reveals that some of these microRNAs (miR-22, miR-214, miR-125a-3p, miR-320 and let-7 family) are associated with reduction of apoptosis. Here, we summarize the roles of key microRNAs associated with IGF-1 in the differentiation of BMSCs into NPCs. These findings may provide clues to further our understanding of the mechanisms and roles of microRNAs as key regulators of BMSC-derived NPC maintenance.
登录
查看更多内容
影响因子：
5.8
作者：
Chen XN;Wang KF;Xu ZQ;Li SJ;Liu Q;Fu DH;Wang X;Wu B
通讯作者：
Wu B
影响因子：
3.7
作者：
Lan X;Chen Q;Wang Y;Jia B;Sun L;Zheng J;Peng H
通讯作者：
Peng H
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
14.9
作者：
Dimmer EC;Huntley RP;Alam-Faruque Y;Sawford T;O'Donovan C;Martin MJ;Bely B;Browne P;Mun Chan W;Eberhardt R;Gardner M;Laiho K;Legge D;Magrane M;Pichler K;Poggioli D;Sehra H;Auchincloss A;Axelsen K;Blatter MC;Boutet E;Braconi-Quintaje S;Breuza L;Bridge A;Coudert E;Estreicher A;Famiglietti L;Ferro-Rojas S;Feuermann M;Gos A;Gruaz-Gumowski N;Hinz U;Hulo C;James J;Jimenez S;Jungo F;Keller G;Lemercier P;Lieberherr D;Masson P;Moinat M;Pedruzzi I;Poux S;Rivoire C;Roechert B;Schneider M;Stutz A;Sundaram S;Tognolli M;Bougueleret L;Argoud-Puy G;Cusin I;Duek-Roggli P;Xenarios I;Apweiler R
通讯作者：
Apweiler R
miR-34a 下调 Bcl-2 表达介导棕榈酸酯诱导的 Min6 细胞凋亡
影响因子：
4.3
作者：
Lin X;Guan H;Huang Z;Liu J;Li H;Wei G;Cao X;Li Y
通讯作者：
Li Y