Progesterone from the cumulus cells is the sperm chemoattractant secreted by the rabbit oocyte cumulus complex.
Progesterone from the cumulus cells is the sperm chemoattractant secreted by the rabbit oocyte cumulus complex.
复制标题
DOI：
10.1371/journal.pone.0003040
复制
发表时间：
2008-08-22
期刊：
影响因子：
3.7
通讯作者：
Giojalas LC
中科院分区：
文献类型：
--
作者：
Guidobaldi HA;Teves ME;Uñates DR;Anastasía A;Giojalas LC
哺乳动物的精子趋化性有几个雌性来源，如卵泡液（FF）、输卵管液、卵积云（CU）和卵母细胞(O)的条件培养基。虽然有几种物质被证实是精子的化学引诱剂，但孕酮(P)似乎是最好的化学引诱剂候选人，因为：1)精子表达细胞表面P受体，2)有能力的精子在体外被低水平的P梯度吸引；3)排卵后CU细胞产生并分泌P；4)沿CU方向P梯度保持稳定；5)精子在体内最可能趋化的位置可能是靠近和/或在CU内。本研究的目的是验证P是否是兔卵丘复合体（OCC）在兔体内分泌的精子趋化剂，作为哺乳动物模型。通过视频显微镜和计算机图像分析，我们观察到只有CU是精子引诱剂的稳定来源。通过免疫细胞化学和酶免疫测定将激素定位于这些细胞内，并在条件培养基中产生和分泌P。此外，兔精子表达一种细胞表面P受体（western blot），并通过免疫细胞化学定位于顶体区域。为了证实P是CU分泌的精子趋化剂，通过三种不同的方法抑制精子对OCC条件培养基的趋化反应：用抗P抗体去除OCC条件培养基中的P，用P反梯度破坏OCC条件培养基的引诱剂梯度，用特异性抗体阻断精子P受体。我们得出结论，只有卵母细胞分泌P，而卵母细胞不分泌P，后者通过细胞表面受体来吸引精子。我们的发现可能对人类、具有经济价值的动物和濒危物种的辅助生殖程序感兴趣。
Sperm chemotaxis in mammals have been identified towards several female sources as follicular fluid (FF), oviduct fluid, and conditioned medium from the cumulus oophorus (CU) and the oocyte (O). Though several substances were confirmed as sperm chemoattractant, Progesterone (P) seems to be the best chemoattractant candidate, because: 1) spermatozoa express a cell surface P receptor, 2) capacitated spermatozoa are chemotactically attracted in vitro by gradients of low quantities of P; 3) the CU cells produce and secrete P after ovulation; 4) a gradient of P may be kept stable along the CU; and 5) the most probable site for sperm chemotaxis in vivo could be near and/or inside the CU. The aim of this study was to verify whether P is the sperm chemoattractant secreted by the rabbit oocyte-cumulus complex (OCC) in the rabbit, as a mammalian animal model. By means of videomicroscopy and computer image analysis we observed that only the CU are a stable source of sperm attractants. The CU produce and secrete P since the hormone was localized inside these cells by immunocytochemistry and in the conditioned medium by enzyme immunoassay. In addition, rabbit spermatozoa express a cell surface P receptor detected by western blot and localized over the acrosomal region by immunocytochemistry. To confirm that P is the sperm chemoattractant secreted by the CU, the sperm chemotactic response towards the OCC conditioned medium was inhibited by three different approaches: P from the OCC conditioned medium was removed with an anti-P antibody, the attractant gradient of the OCC conditioned medium was disrupted by a P counter gradient, and the sperm P receptor was blocked with a specific antibody. We concluded that only the CU but not the oocyte secretes P, and the latter chemoattract spermatozoa by means of a cell surface receptor. Our findings may be of interest in assisted reproduction procedures in humans, animals of economic importance and endangered species.
登录
查看更多内容
影响因子：
3.6
作者：
HARPER, MJK
通讯作者：
HARPER, MJK
影响因子：
--
作者：
HAUSER, R;HOMONNAI, ZT;YOGEV, L
通讯作者：
YOGEV, L
DOI：
10.1530/reprod/118.1.145
发表时间：
2000-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
Pintado, B;de la Fuente, J;Roldan, ERS
通讯作者：
Roldan, ERS
影响因子：
6.7
作者：
Chian, RC;Ao, A;Tan, SL
通讯作者：
Tan, SL
影响因子：
6.1
作者：
Calogero, AE;Burrello, N;D'Agata, R
通讯作者：
D'Agata, R