Rbm24a and Rbm24b are required for normal somitogenesis.
Rbm24a and Rbm24b are required for normal somitogenesis.
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Rbm24a 和 Rbm24b 是正常体细胞发生所必需的。
DOI：
10.1371/journal.pone.0105460
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
McCallion AS
中科院分区：
文献类型：
--
作者：
Maragh S;Miller RA;Bessling SL;Wang G;Hook PW;McCallion AS
我们最近证明了编码RNA结合基序蛋白24（RBM 24）的基因在小鼠心脏发生过程中表达，并确定了其斑马鱼同源物Rbm 24 a和Rbm 24 b在心脏发育过程中的发育要求。我们在这里证明，Rbm 24 a和Rbm 24 b也需要正常的体节和颅面发育。减少rbm 24 a或rbm 24 b基因产物的吗啉敲除导致显着的体节形成中断。详细的原位杂交为基础的分析，一系列的somitogenesis-associated转录显示减少表达的周期性肌肉模式基因dlc和dld编码的Notch配体，以及它们各自的靶基因her 7，her 1。相比之下，Notch受体notch 1a和notch 3的表达似乎没有变化。一些RBM家族成员参与前mRNA加工。对rbm 24 a和rbm 24 b注射吗啡的胚胎中受影响的Notch途径mRNA的分析显示dlc和dld的异常转录片段，但没有her 1或her 7，这表明Notch途径组分转录水平的降低可能是由于其配体的异常加工造成的。这些数据意味着一个以前未知的需求Rbm 24 a和Rbm 24 b体节和颅面发育。虽然我们预期破坏RBM 24同源物的影响可能超出Notch途径，但我们的结果表明它们的扰动可能直接或间接地损害转录后加工，例如dlc和dld的不精确加工。
We recently demonstrated that the gene encoding the RNA binding motif protein 24 (RBM24) is expressed during mouse cardiogenesis, and determined the developmental requirement for its zebrafish homologs Rbm24a and Rbm24b during cardiac development. We demonstrate here that both Rbm24a and Rbm24b are also required for normal somite and craniofacial development. Diminution of rbm24a or rbm24b gene products by morpholino knockdown resulted in significant disruption of somite formation. Detailed in situ hybridization-based analyses of a spectrum of somitogenesis-associated transcripts revealed reduced expression of the cyclic muscle pattering genes dlc and dld encoding Notch ligands, as well as their respective target genes her7, her1. By contrast expression of the Notch receptors notch1a and notch3 appears unchanged. Some RBM-family members have been implicated in pre-mRNA processing. Analysis of affected Notch-pathway mRNAs in rbm24a and rbm24b morpholino-injected embryos revealed aberrant transcript fragments of dlc and dld, but not her1 or her7, suggesting the reduction in transcription levels of Notch pathway components may result from aberrant processing of its ligands. These data imply a previously unknown requirement for Rbm24a and Rbm24b in somite and craniofacial development. Although we anticipate the influence of disrupting RBM24 homologs likely extends beyond the Notch pathway, our results suggest their perturbation may directly, or indirectly, compromise post-transcriptional processing, exemplified by imprecise processing of dlc and dld.
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影响因子：
64.8
作者：
Jiang, YJ;Aerne, BL;Lewis, J
通讯作者：
Lewis, J
DOI：
10.1083/jcb.201204138
发表时间：
2012-10-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Jayasena CS;Bronner ME
通讯作者：
Bronner ME
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
2.7
作者：
Jülich, D;Lim, CH;Holley, SA
通讯作者：
Holley, SA
影响因子：
3.5
作者：
Jin, Wenxing;Niu, Zhaoyang;Li, Xialu
通讯作者：
Li, Xialu