Comparison of quantum dot technology with conventional immunohistochemistry in examining aldehyde dehydrogenase 1A1 as a potential biomarker for lymph node metastasis of head and neck cancer.
Comparison of quantum dot technology with conventional immunohistochemistry in examining aldehyde dehydrogenase 1A1 as a potential biomarker for lymph node metastasis of head and neck cancer.
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DOI：
10.1016/j.ejca.2011.12.029
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发表时间：
2012-07
影响因子：
8.4
通讯作者：
Chen, Zhuo (Georgia)
中科院分区：
文献类型：
--
作者：
Xu, Jing;Mueller, Susan;Nannapaneni, Sreenivas;Pan, Lin;Wang, Yuxiang;Peng, Xianghong;Wang, Dongsheng;Tighiouart, Mourad;Chen, Zhengjia;Saba, Nabil F.;Beitler, Jonathan J.;Shin, Dong M.;Chen, Zhuo (Georgia)
关键词：
本研究探讨乙醛脱氢酶1（ALDH 1A 1）在头颈部鳞状细胞癌（HNSCC）原发灶中的表达与淋巴结转移（LNM）的关系。我们使用量子点（QD）为基础的荧光（IHF）和传统的免疫组织化学（IHC），以定量ALDH 1A 1表达的原发性肿瘤样本取自96 HNSCC患者，50与疾病的淋巴结和46没有。采用单因素和多因素分析评价ALDH 1A 1定量表达水平与HNSCC患者LNM的相关性。采用Kaplan-Meier分析和Wald检验分析ALDH 1A 1的预后价值。ALDH 1A 1与HNSCC患者的LNM高度相关（基于QD的IHF的p < 0.0001，IHC的p <0.039）。发现用于定量ALDH 1A 1的两种方法（基于QD的IHF和常规IHC）相当（R = 0.75，p < 0.0001），但QD-IHF比IHC更敏感和客观。ALDH 1A 1低表达的HNSCC患者的5年生存率高于ALDH 1A 1高表达的患者（p = 0.025）。我们的研究表明，ALDH 1A 1是预测HNSCC患者LNM的潜在生物标志物，尽管它不是HNSCC患者生存的独立预后因素。此外，QD-IHF在定量HNSCC组织中ALDH 1A 1表达方面优于IHC。
This study explored whether expression of aldehyde dehydrogenase 1 (ALDH1A1) in the primary tumor correlated with lymph node metastasis (LNM) of squamous cell carcinoma of the head and neck (HNSCC). We used both quantum dot (QD)-based immunohistofluorescence (IHF) and conventional immunohistochemistry (IHC) to quantify ALDH1A1 expression in primary tumor samples taken from 96 HNSCC patients, 50 with disease in the lymph nodes and 46 without. The correlation between the quantified level of ALDH1A1 expression and LNM in HNSCC patients was evaluated with univariate and multivariate analysis. The prognostic value of ALDH1A1 was examined by Kaplan-Meier analysis and Wald test. ALDH1A1 was highly correlated with LNM in HNSCC patients (p < 0.0001 by QD-based IHF and 0.039 by IHC). The two methods (QD-based IHF and conventional IHC) for quantification of ALDH1A1 were found to be comparable (R = 0.75, p < 0.0001), but QD-IHF was more sensitive and objective than IHC. The HNSCC patients with low ALDH1A1 expression had a higher 5-year survival rate than those with high ALDH1A1 level (p = 0.025). Our study suggests that ALDH1A1 is a potential biomarker for predicting LNM in HNSCC patients, though it is not an independent prognostic factor for survival of HNSCC patients. Furthermore, QD-IHF has advantages over IHC in quantification of ALDH1A1 expression in HNSCC tissues.
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影响因子：
254.7
作者：
Parkin, DM;Bray, F;Pisani, P
通讯作者：
Pisani, P
影响因子：
56.9
作者：
Bruchez, M;Moronne, M;Alivisatos, AP
通讯作者：
Alivisatos, AP
DOI：
10.1016/j.bbrc.2009.05.048
发表时间：
2009-07-31
影响因子：
3.1
作者：
Chen, Yu-Chih;Chen, Yi-Wei;Lo, Wen-Liang
通讯作者：
Lo, Wen-Liang
影响因子：
5.2
作者：
Ma, Stephanie;Chan, Kwok Wah;Guan, Xin-Yuan
通讯作者：
Guan, Xin-Yuan
DOI：
10.1056/nejmoa0912217
发表时间：
2010-07-01
期刊：
The New England journal of medicine
影响因子：
--
作者：
Ang KK;Harris J;Wheeler R;Weber R;Rosenthal DI;Nguyen-Tân PF;Westra WH;Chung CH;Jordan RC;Lu C;Kim H;Axelrod R;Silverman CC;Redmond KP;Gillison ML
通讯作者：
Gillison ML