cDNA-AFLP analysis of plant and pathogen genes expressed in grapevine infected with Plasmopara viticola.
cDNA-AFLP analysis of plant and pathogen genes expressed in grapevine infected with Plasmopara viticola.
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DOI：
10.1186/1471-2164-9-142
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发表时间：
2008-03-26
期刊：
影响因子：
4.4
通讯作者：
Polverari A
中科院分区：
文献类型：
--
作者：
Polesani M;Desario F;Ferrarini A;Zamboni A;Pezzotti M;Kortekamp A;Polverari A
卵菌Plasmopara viticola（Berk.（Curt）Berl.和de Toni引起葡萄（葡萄属葡萄属L.）的霜霉病。这种病原体是严格的活体营养型，因此完全依赖于活的宿主细胞生存。该系统中的相容性和疾病发展的分子基础知之甚少。我们进行了大规模的cDNA-AFLP分析，以鉴定与感染过程相关的葡萄和葡萄疫霉基因。我们进行了cDNA-AFLP分析，在油斑期的敏感品种雷司令人工感染的叶片，水处理的叶片和作为对照的纯孢子囊样品。选择性扩增与128个引物组合允许可视化的约7000个转录衍生片段（TDFs）在感染的叶子，其中1196（17%）差异表达。我们对984个片段进行了测序，其中804个片段经同源性搜索后被鉴定为葡萄转录本，而96个片段与疫霉属的序列同源。数据库，并归因于P. viticola。有82个孤儿TDF。许多葡萄基因几乎涵盖所有功能类别在感染过程中下调，特别是参与光合作用的基因。与已知抗性基因同源的葡萄藤基因也倾向于被抑制，如几个抗性基因类似物和碳酸酐酶（最近与病原体抗性有关）。相反，编码细胞骨架成分、苯丙素酶和β-氧化途径以及发病相关蛋白的基因在感染期间主要上调。葡萄疫霉在植物中表达的大部分转录本与功能未知的基因或疫霉基因组序列具有同源性，但也鉴定了与代谢、能量产生、运输和信号转导相关的基因。这项研究提供了第一个全球性的葡萄和葡萄疫霉感染过程中表达的基因目录，以及它们的功能注释。这将有助于阐明感染过程的分子基础，并确定有助于抑制病原体的基因和化学物质。
The oomycete Plasmopara viticola (Berk. and Curt.) Berl. and de Toni causes downy mildew in grapevine (Vitis vinifera L.). This pathogen is strictly biotrophic, thus completely dependent on living host cells for its survival. The molecular basis of compatibility and disease development in this system is poorly understood. We have carried out a large-scale cDNA-AFLP analysis to identify grapevine and P. viticola genes associated with the infection process. We carried out cDNA-AFLP analysis on artificially infected leaves of the susceptible cultivar Riesling at the oil spot stage, on water-treated leaves and on a sample of pure sporangia as controls. Selective amplifications with 128 primer combinations allowed the visualization of about 7000 transcript-derived fragments (TDFs) in infected leaves, 1196 of which (17%) were differentially expressed. We sequenced 984 fragments, 804 of which were identified as grapevine transcripts after homology searching, while 96 were homologous to sequences in Phytophthora spp. databases and were attributed to P. viticola. There were 82 orphan TDFs. Many grapevine genes spanning almost all functional categories were downregulated during infection, especially genes involved in photosynthesis. Grapevine genes homologous to known resistance genes also tended to be repressed, as were several resistance gene analogs and carbonic anhydrase (recently implicated in pathogen resistance). In contrast, genes encoding cytoskeletal components, enzymes of the phenylpropanoid and beta-oxidation pathways, and pathogenesis related proteins were primarily upregulated during infection. The majority of P. viticola transcripts expressed in planta showed homology to genes of unknown function or to genomic Phytophthora sequences, but genes related to metabolism, energy production, transport and signal transduction were also identified. This study provides the first global catalogue of grapevine and P. viticola genes expressed during infection, together with their functional annotations. This will help to elucidate the molecular basis of the infection process and identify genes and chemicals that could help to inhibit the pathogen.
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影响因子：
7.2
作者：
Bachem, CWB;vanderHoeven, RS;Visser, RGF
通讯作者：
Visser, RGF
影响因子：
5.4
作者：
Doligez, A.;Adam-Blondon, A. F.;Di Gaspero, G.
通讯作者：
Di Gaspero, G.
影响因子：
64.8
作者：
Jaillon, Olivier;Aury, Jean-Marc;Wincker, Patrick
通讯作者：
Wincker, Patrick
DOI：
10.1111/j.1749-6632.1984.tb12340.x
发表时间：
1984-01-01
影响因子：
5.2
作者：
GRAHAM, D;REED, ML;DWYER, MR
通讯作者：
DWYER, MR
影响因子：
4.3
作者：
Jin, SM;Xu, RL;Goodwin, PH
通讯作者：
Goodwin, PH