A tomato HD-zip I transcription factor, VAHOX1, acts as a negative regulator of fruit ripening.
A tomato HD-zip I transcription factor, VAHOX1, acts as a negative regulator of fruit ripening.
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番茄 HD-Zip I 转录因子 VAHOX1 作为果实成熟的负调节因子
DOI：
10.1093/hr/uhac236
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发表时间：
2023
影响因子：
8.7
通讯作者：
中科院分区：
文献类型：
--
作者：
同源结构域亮氨酸拉链（HD-Zip）转录因子仅存在于高等植物中，并参与植物发育和胁迫反应。然而，我们对它们参与经济植物如番茄（Solanum lycopersicum）果实成熟的理解仍然很不清楚。在这里，我们报告，VAHOX 1，番茄HD-Zip I亚家族的成员，在所有组织中表达，在断路器+4果实中高度表达，并可被乙烯诱导。VAHOX 1的RNAi抑制（VAHOX 1-RNAi）导致果实成熟加速，对乙烯的敏感性增强，总类胡萝卜素含量和乙烯产量增加。相反，番茄中VAHOX 1过表达（VAHOX 1-OE）具有相反的效果。RNA-Seq结果表明，改变VAHOX 1的表达影响了一系列参与乙烯生物合成和信号转导以及细胞壁修饰的基因的转录积累。此外，双荧光素酶报告基因检测、GUS活性组织化学分析和酵母单杂交（Y1 H）检测表明VAHOX 1可以激活AP 2a的表达。本研究的结果有助于我们进一步了解番茄中HD-Zip转录因子的生理功能，并揭示番茄果实成熟过程中转录调控的多样性。
Homeodomain-leucine zipper (HD-Zip) transcription factors are only present in higher plants and are involved in plant development and stress responses. However, our understanding of their participation in the fruit ripening of economical plants, such as tomato (Solanum lycopersicum), remains largely unclear. Here, we report that VAHOX1, a member of the tomato HD-Zip I subfamily, was expressed in all tissues, was highly expressed in breaker+4 fruits, and could be induced by ethylene. RNAi repression of VAHOX1 (VAHOX1-RNAi) resulted in accelerated fruit ripening, enhanced sensitivity to ethylene, and increased total carotenoid content and ethylene production. Conversely, VAHOX1 overexpression (VAHOX1-OE) in tomato had the opposite effect. RNA-Seq results showed that altering VAHOX1 expression affected the transcript accumulation of a series of genes involved in ethylene biosynthesis and signal transduction and cell wall modification. Additionally, a dual-luciferase reporter assay, histochemical analysis of GUS activity and a yeast one-hybrid (Y1H) assay revealed that VAHOX1 could activate the expression of AP2a. Our findings may expand our knowledge about the physiological functions of HD-Zip transcription factors in tomato and highlight the diversities of transcriptional regulation during the fruit ripening process.
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