Increased percentages of T helper cells producing IL-17 and monocytes expressing markers of alternative activation in patients with sepsis.
Increased percentages of T helper cells producing IL-17 and monocytes expressing markers of alternative activation in patients with sepsis.
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DOI：
10.1371/journal.pone.0037393
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Salomao R
中科院分区：
文献类型：
--
作者：
Brunialti MK;Santos MC;Rigato O;Machado FR;Silva E;Salomao R
据报道，脓毒症患者（SP）从Th 1向Th 2转变，以及Treg CD 4 +T细胞亚群增加。此外，这些患者表现出单核细胞功能的调节，在LPS刺激后促炎细胞因子的产生减少，这类似于交替活化的巨噬细胞的表型。在这项研究中，我们评估了T细胞分化为Th 1，Th 17和Treg亚群的百分比，以及单核细胞表达交替激活的单核细胞/巨噬细胞（AAM）的标志物的百分比在SP。外周血单核细胞（PBMC）从32名健康志愿者（HV）和SP在入院时（D 0，n = 67）和治疗7天后（D 7，n = 33）。 Th 1和Th 17（CD 3 + CD 8 −）淋巴细胞通过IFN-γ和IL-17的细胞内检测分别自发和PMA/Io刺激后鉴定，Treg细胞通过Foxp 3 + CD 127-表达鉴定。评价单核细胞的CD 206和CD 163表达。通过流式细胞术测量全血样品中的CD 4 +T淋巴细胞的绝对数。适当时应用Mann-Whitney或Wilcoxon检验。PMA/Io刺激后，SP组Th 1细胞百分比低于HV组，而Th 17细胞百分比高于HV组。在患者的随访样本中，与D 0样本相比，在D 7观察到更高百分比的Th 1细胞和更低百分比的Th 17细胞。Treg细胞保持不变。脓毒症患者显示表达CD 163和CD 206的单核细胞比例显著增加。在体外刺激下，在入院时SP中产生IL-17的T辅助淋巴细胞的百分比高于HV，而产生IFN-γ的百分比较低，这种模式在随访期间逆转。CD 163和CD 206的表达增加表明单核细胞在脓毒症期间可获得AAM表型。
A shift from Th1 to Th2 as well as an increase in Treg CD4+T cell subsets has been reported in septic patients (SP). Furthermore, these patients display modulation of monocyte function, with reduced production of pro-inflammatory cytokines upon LPS stimulus, which resembles the phenotype of alternatively activated macrophages. In this study, we evaluated the percentages of T cells differentiated into Th1, Th17 and Treg subsets, as well as the percentage of monocytes expressing markers of alternatively activated monocytes/macrophages (AAM) in SP. Peripheral blood mononuclear cells (PBMC) were obtained from 32 healthy volunteers (HV) and from SP at admission (D0, n = 67) and after 7 days of therapy (D7, n = 33). Th1 and Th17 (CD3+CD8−) lymphocytes were identified by the intracellular detection of IFN-γ and IL-17, respectively, spontaneously and after PMA/Io stimulation, and Treg cells were identified by Foxp3+CD127- expression. Monocytes were evaluated for CD206 and CD163 expression. Absolute numbers of CD4+T lymphocytes were measured in whole blood samples by flow cytometry. The Mann-Whitney or Wilcoxon test was applied, as appropriate. The percentage of Th1 cells was lower in SP than in HV at admission after PMA/Io stimulation, whereas the percentage of Th17 cells was higher. In patients’ follow-up samples, a higher percentage of Th1 cells and a lower percentage of Th17 cells were observed on D7 compared with the D0 samples. Treg cells remained unchanged. Septic patients showed a markedly increased proportion of monocytes expressing CD163 and CD206. Upon in vitro stimulus, the percentage of T helper lymphocytes producing IL-17 was higher in SP than in HV at admission, and the percentage producing IFN-γ was lower, a pattern that was reversed during follow-up. The increased expression of CD163 and CD206 indicates that monocytes may acquire the AAM phenotype during sepsis.
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影响因子：
64.8
作者：
Bettelli, E;Carrier, YJ;Kuchroo, VK
通讯作者：
Kuchroo, VK
CD127表达与FOXP3和人类CD4+ T Reg细胞的抑制功能成反比。
DOI：
10.1084/jem.20060772
发表时间：
2006-07-10
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1186/cc5055
发表时间：
2006
期刊：
Critical care (London, England)
影响因子：
--
作者：
Cavaillon JM;Adib-Conquy M
通讯作者：
Adib-Conquy M
影响因子：
4.8
作者：
Flierl, Michael A.;Rittirsch, Daniel;Ward, Peter A.
通讯作者：
Ward, Peter A.
DOI：
10.1186/cc8876
发表时间：
2010
期刊：
Critical care (London, England)
影响因子：
--
作者：
Hein F;Massin F;Cravoisy-Popovic A;Barraud D;Levy B;Bollaert PE;Gibot S
通讯作者：
Gibot S