Oxidant stress evoked damage in rat hepatocyte leading to triggered nitric oxide synthase (NOS) levels on long term consumption of aspartame.
Oxidant stress evoked damage in rat hepatocyte leading to triggered nitric oxide synthase (NOS) levels on long term consumption of aspartame.
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DOI：
10.1016/j.jfda.2014.07.011
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发表时间：
2015-12
影响因子：
3.6
通讯作者：
Sheeladevi, Rathinasamy
中科院分区：
文献类型：
--
作者：
Ashok, Iyaswamy;Sheeladevi, Rathinasamy
本研究探讨了长期（40 mg/kg B.wt）摄入西莫司如何改变Wistar白化病大鼠肝脏的抗氧化状态、应激途径基因和凋亡变化。许多有争议的报告是关于使用阿替西泮，因为它在代谢过程中释放甲醇作为其代谢产物之一。为了模拟人类甲醇代谢，纳入甲氨蝶呤处理的大鼠以研究苯丙氨酸甲酯的作用。甲氨蝶呤（MTX）处理的动物中，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、脂质过氧化（LPO）和谷胱甘肽过氧化物酶（GPx）活性在对照组和MTX对照组动物的肝脏中显著增加，并且在甲氨蝶呤处理的动物中，还原型谷胱甘肽（GSH）和蛋白巯基显著降低。阿贝沙坦处理的MTX动物显示体重、脑和肝重量显著降低。在对照组和MTX对照组动物的肝脏中，阿糖胞苷处理的MTX动物显示诱导型一氧化氮（iNOS）、神经元型一氧化氮（nNOS）、c-fos、热休克蛋白（Hsp）70、肿瘤坏死因子（TNF）α、半胱天冬酶8、c-jun N末端激酶（JNK）3和核因子κ B（NF κ B）基因表达显著增加。在对照组和MTX对照组动物的肝脏中，经阿糖胞苷处理的MTX动物显示出c-fos、Hsp 70、iNOS Caspase 8和JNK 3蛋白表达的显著增加，表明应激和细胞凋亡的增强。阿糖胞苷处理的MTX动物在肝脏中显示出一条明显的DNA片段。在免疫组织化学分析中，替吉奥处理的动物显示棕色阳性肝细胞，表明应激特异性和凋亡蛋白表达。由于人们对甜味剂的消费量正在上升，因此必须提高对这种人造甜味剂使用的认识。
This study investigates how long-term (40 mg/kg b.wt) consumption of aspartame can alter the antioxidant status, stress pathway genes, and apoptotic changes in the liver of Wistar albino rats. Numerous controversial reports are available on the use of aspartame as it releases methanol as one of its metabolites during metabolism. To mimic the human methanol metabolism the methotrexate treated rats were included to study the aspartame effects. The aspartame treated methotrexate (MTX animals showed a marked significant increase in the superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (LPO), and Glutathione peroxidase (GPx) activity in the liver from control and MTX control animals, and showed a significant decrease in reduced glutathione (GSH) and protein thiol in aspartame treated animals. The aspartame treated MTX animals showed a marked significant decrease in the body weight, brain, and liver weight. The aspartame treated MTX animals showed a marked increase in the inducible nitric oxide (iNOS), neuronal nitric oxide (nNOS), c-fos, Heat shock protein (Hsp) 70 Tumour necrosis Factor (TNF)α, caspase 8, c-jun N terminal kinases (JNK) 3 and Nuclear factor kappa B (NFkB) gene expression in the liver from control and MTX control animals. The aspartame treated MTX animals showed a marked increase in the c-fos, Hsp 70, iNOS Caspase 8, and JNK 3 protein expression in the liver from control and MTX control animals indicating the enhancement of stress and apoptosis. The aspartame treated MTX animals showed a streak of marked DNA fragmentation in the liver. On immunohistochemical analysis aspartame treated animals showed brown colored positive hepatocytes indicating the stress specific and apoptotic protein expression. Since aspartame consumption is on the rise among people, it is essential to create awareness regarding the usage of this artificial sweetener.
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影响因子：
3.8
作者：
Chang, YF;Chi, CW;Wang, JJ
通讯作者：
Wang, JJ
影响因子：
4.3
作者：
Bandyopadhyay S;Kelley R;Krogan NJ;Ideker T
通讯作者：
Ideker T
影响因子：
3.8
作者：
González-Quevedo, A;Obregón, F;Lima, L
通讯作者：
Lima, L
DOI：
10.1080/15287399409531827
发表时间：
1994-01-01
期刊：
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH
影响因子：
--
作者：
LEE, EW;GARNER, CD;TERZO, TS
通讯作者：
TERZO, TS
影响因子：
2.8
作者：
Gurel, A;Coskun, O;Ozen, OA
通讯作者：
Ozen, OA