Regulation of extracellular matrix elements and sarcomerogenesis in response to different periods of passive stretching in the soleus muscle of rats.
Regulation of extracellular matrix elements and sarcomerogenesis in response to different periods of passive stretching in the soleus muscle of rats.
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DOI：
10.1038/s41598-018-27239-x
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发表时间：
2018-06-13
影响因子：
4.6
通讯作者：
Salvini TF
中科院分区：
文献类型：
--
作者：
Peviani SM;Guzzoni V;Pinheiro-Dardis CM;Silva YPD;Fioravante ACR;Sagawa AH;Delfino GB;Durigan JLQ;Salvini TF
拉伸是防止肌肉缩短和改善活动受限的常用方法。然而，不同时间段对拉伸诱导的细胞外基质及其调节元件的适应的影响仍有待研究。我们的目的是评估不同拉伸期大鼠比目鱼肌 (SOL) 中纤维状胶原蛋白的表达、肌瘤生成、金属蛋白酶 (MMP) 活性和细胞外基质 (ECM) 调节剂的基因表达。在 10 天（St 10d）或 15 天（St 15d）内对比目鱼肌进行 10 组被动拉伸（每组 1 分钟，组间休息 30 秒）。分析了肌瘤生成、肌肉横截面积 (CSA)、MMP 活性以及胶原蛋白（I、III 和 IV 型）、结缔组织生长因子 (CTGF)、生长因子-β (TGF-β) 和赖氨酰氧化酶 (LOX) 中的 mRNA 水平。这两个时间段的被动拉伸均减轻了大鼠 SOL 肌肉中的 COL-I 沉积。矛盾的是，10 天的被动拉伸诱导了 COL-I 和 COL-III 的合成，同时在转录水平上上调了 TGF-β1 和 CTGF。这些反应可能与接受 10 次被动拉伸训练的 SOL 肌肉中较低的 LOX mRNA 水平有关。此外，在拉伸 15 天后观察到肌瘤生成，这表明拉伸引起的肌肉适应是时间依赖性反应。
Stretching is a common method used to prevent muscle shortening and improve limited mobility. However, the effect of different time periods on stretching-induced adaptation of the extracellular matrix and its regulatory elements have yet to be investigated. We aimed to evaluate the expression of fibrillar collagens, sarcomerogenesis, metalloproteinase (MMP) activity and gene expression of the extracellular matrix (ECM) regulators in the soleus (SOL) muscle of rats submitted to different stretching periods. The soleus muscles were submitted to 10 sets of passive stretching over 10 (St 10d) or 15 days (St 15d) (1 min per set, with 30 seconds’ rest between sets). Sarcomerogenesis, muscle cross-sectional area (CSA), and MMP activity and mRNA levels in collagen (type I, III and IV), connective tissue growth factor (CTGF), growth factor-beta (TGF-β), and lysyl oxidase (LOX) were analyzed. Passive stretching over both time periods mitigated COL-I deposition in the SOL muscle of rats. Paradoxically, 10 days of passive stretching induced COL-I and COL-III synthesis, with concomitant upregulation of TGF-β1 and CTGF at a transcriptional level. These responses may be associated with lower LOX mRNA levels in SOL muscles submitted to 10 passive stretching sessions. Moreover, sarcomerogenesis was observed after 15 days of stretching, suggesting that stretching-induced muscle adaptations are time-dependent responses.
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影响因子：
4
作者：
Kagan, HM;Li, WD
通讯作者：
Li, WD
DOI：
10.1016/s0003-9993(03)00406-4
发表时间：
2004-01-01
影响因子：
4.3
作者：
Ikeda, S;Yoshida, A;Tanaka, N
通讯作者：
Tanaka, N
DOI：
10.1152/ajpregu.2001.280.5.r1292
发表时间：
2001-05-01
影响因子：
2.8
作者：
Koskinen, SOA;Wang, W;Takala, TES
通讯作者：
Takala, TES
影响因子：
3.4
作者：
Gillies, Allison R.;Lieber, Richard L.
通讯作者：
Lieber, Richard L.
影响因子：
5.5
作者：
Heinemeier, K. M.;Olesen, J. L.;Schjerling, P.
通讯作者：
Schjerling, P.