New mass-spectrometry-compatible degradable surfactant for tissue proteomics.
New mass-spectrometry-compatible degradable surfactant for tissue proteomics.
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DOI：
10.1021/pr5012679
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发表时间：
2015-03-06
影响因子：
4.4
通讯作者：
Ge, Ying
中科院分区：
文献类型：
--
作者：
Chang, Ying-Hua;Gregorich, Zachery R.;Chen, Albert J.;Hwang, Leekyoung;Guner, Huseyin;Yu, Deyang;Zhang, Jianyi;Ge, Ying
组织蛋白质组学在生物标志物发现和疾病机制研究中的作用日益受到重视。然而，蛋白质溶解度仍然是基于质谱（MS）的组织蛋白质组学中的重大挑战。常规的表面活性剂，如十二烷基硫酸钠（SDS），蛋白质增溶的优选表面活性剂，是不兼容的MS。在这里，我们已经筛选了一个库的表面活性剂样化合物，并发现了MS兼容的可降解的表面活性剂（MaSDeS）的组织蛋白质组学，溶解所有类别的蛋白质与SDS的性能相当。在组织提取中使用MaSDeS显著提高了从常用组织（包括来自心脏、肝脏和肺的组织）中鉴定蛋白质的总数。值得注意的是，MaSDeS显着丰富的膜蛋白，这往往是在蛋白质组学研究的代表性不足。MaSDeS的酸可降解性质使其适合于基于高通量质谱的蛋白质组学。此外，MaSDeS的热稳定性允许其在需要高温的实验中使用，以促进蛋白质提取和溶解。此外，我们已经表明，MaSDeS优于其他MS兼容的表面活性剂在整体蛋白质溶解度和组织蛋白质组学中鉴定的蛋白质的总数。因此，MaSDeS的使用将大大推进组织蛋白质组学，并实现其在基础生物医学和临床研究中的潜力。MaSDeS可用于各种蛋白质组学研究以及采用表面活性剂溶解蛋白质的一般生化和生物实验。
Tissue proteomics is increasingly recognized for its role in biomarker discovery and disease mechanism investigation. However, protein solubility remains a significant challenge in mass spectrometry (MS)-based tissue proteomics. Conventional surfactants such as sodium dodecyl sulfate (SDS), the preferred surfactant for protein solubilization, are not compatible with MS. Herein, we have screened a library of surfactant-like compounds and discovered an MS-compatible degradable surfactant (MaSDeS) for tissue proteomics that solubilizes all categories of proteins with performance comparable to SDS. The use of MaSDeS in the tissue extraction significantly improves the total number of protein identifications from commonly used tissues, including tissue from the heart, liver, and lung. Notably, MaSDeS significantly enriches membrane proteins, which are often under-represented in proteomics studies. The acid degradable nature of MaSDeS makes it amenable for high-throughput mass spectrometry-based proteomics. In addition, the thermostability of MaSDeS allows for its use in experiments requiring high temperature to facilitate protein extraction and solubilization. Furthermore, we have shown that MaSDeS outperforms the other MS-compatible surfactants in terms of overall protein solubility and the total number of identified proteins in tissue proteomics. Thus, the use of MaSDeS will greatly advance tissue proteomics and realize its potential in basic biomedical and clinical research. MaSDeS could be utilized in a variety of proteomics studies as well as general biochemical and biological experiments that employ surfactants for protein solubilization.
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影响因子：
64.8
作者：
Kim, Min-Sik;Pinto, Sneha M.;Getnet, Derese;Nirujogi, Raja Sekhar;Manda, Srikanth S.;Chaerkady, Raghothama;Madugundu, Anil K.;Kelkar, Dhanashree S.;Isserlin, Ruth;Jain, Shobhit;Thomas, Joji K.;Muthusamy, Babylakshmi;Leal-Rojas, Pamela;Kumar, Praveen;Sahasrabuddhe, Nandini A.;Balakrishnan, Lavanya;Advani, Jayshree;George, Bijesh;Renuse, Santosh;Selvan, Lakshmi Dhevi N.;Patil, Arun H.;Nanjappa, Vishalakshi;Radhakrishnan, Aneesha;Prasad, Samarjeet;Subbannayya, Tejaswini;Raju, Rajesh;Kumar, Manish;Sreenivasamurthy, Sreelakshmi K.;Marimuthu, Arivusudar;Sathe, Gajanan J.;Chavan, Sandip;Datta, Keshava K.;Subbannayya, Yashwanth;Sahu, Apeksha;Yelamanchi, Soujanya D.;Jayaram, Savita;Rajagopalan, Pavithra;Sharma, Jyoti;Murthy, Krishna R.;Syed, Nazia;Goel, Renu;Khan, Aafaque A.;Ahmad, Sartaj;Dey, Gourav;Mudgal, Keshav;Chatterjee, Aditi;Huang, Tai-Chung;Zhong, Jun;Wu, Xinyan;Shaw, Patrick G.;Freed, Donald;Zahari, Muhammad S.;Mukherjee, Kanchan K.;Shankar, Subramanian;Mahadevan, Anita;Lam, Henry;Mitchell, Christopher J.;Shankar, Susarla Krishna;Satishchandra, Parthasarathy;Schroeder, John T.;Sirdeshmukh, Ravi;Maitra, Anirban;Leach, Steven D.;Drake, Charles G.;Halushka, Marc K.;Prasad, T. S. Keshava;Hruban, Ralph H.;Kerr, Candace L.;Bader, Gary D.;Iacobuzio-Donahue, Christine A.;Gowda, Harsha;Pandey, Akhilesh
通讯作者：
Pandey, Akhilesh
完整膜蛋白复合物的质谱法。
影响因子：
14.8
作者：
通讯作者：
--
影响因子：
3.4
作者：
Bereman, Michael S.;Egertson, Jarrett D.;MacCoss, Michael J.
通讯作者：
MacCoss, Michael J.
DOI：
10.1073/pnas.0510977103
发表时间：
2006-05-16
影响因子：
11.1
作者：
Kussmaul, Lothar;Hirst, Judy
通讯作者：
Hirst, Judy
影响因子：
4.4
作者：
Collins, J. W.;Coldham, N. G.;La Ragione, R. M.
通讯作者：
La Ragione, R. M.