Vascular tissue engineering with magnetic nanoparticles: seeing deeper
Vascular tissue engineering with magnetic nanoparticles: seeing deeper
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使用磁性纳米颗粒的血管组织工程：看得更深入
DOI：
10.1002/term.32
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发表时间：
2007
影响因子：
3.3
通讯作者：
E. Wintermantel
中科院分区：
文献类型：
--
作者：
H. Perea;J. Aigner;J. Heverhagen;U. Hopfner;E. Wintermantel
血管内皮细胞在血管疾病中具有重要的治疗和诊断意义。目前的工作提出了一种新的策略，基于使用超顺磁性纳米颗粒来获得血管管腔表面的内皮细胞衬里，可以在临床磁共振成像(MRI)扫描仪中进行非侵入性检测。用临床批准的超顺磁性纳米颗粒预先标记人脐静脉内皮细胞(HUVECs)。细胞存活率和eNOS表达不受标记程序的影响。磁性标记的细胞通过定制的电磁铁输送到聚四氟乙烯管状移植物的管腔上。在1，5T磁共振扫描仪上检测内皮细胞。磁性细胞输送提供了一种高效的技术来种植管状支架，使得能够从基质中非侵入性地描绘细胞，从而为评估细胞输送过程的质量提供了可靠的工具。版权所有©2007 John Wiley&Sons，Ltd.
The endothelium bares a paramount therapeutic and diagnostic significance in vascular disease. The current work presents a novel strategy based on the use of superparamagnetic nanoparticles to obtain an endothelial cell lining on the luminal surface of vascular conduits, which can be detected non‐invasively in a clinical Magnetic Resonance Imaging (MRI) scanner. Human umbilical vein endothelial cells (HUVECs) were prelabeled with clinically approved superparamagnetic nanoparticles. Cell viability and eNOS expression were not affected by the labelling procedure. Magnetically labelled cells were delivered onto the lumen of a PTFE tubular graft by a customised electromagnet. The endothelium was detected in a 1,5T MRI scanner. Magnetic cell delivery provides an efficient technique to seed tubular scaffolds enabling the non‐invasive depiction of the cells from the substrate, thus providing a reliable tool to assess the quality of cell delivery procedures. Copyright © 2007 John Wiley & Sons, Ltd.
影响因子：
16.1
作者：
S. Parikh;E. Edelman;E. Edelman
通讯作者：
S. Parikh;E. Edelman;E. Edelman
影响因子：
56.9
作者：
Niklason, LE;Gao, J;Langer, R
通讯作者：
Langer, R