Multicolor stimulated Raman scattering (SRS) microscopy.

Multicolor stimulated Raman scattering (SRS) microscopy.
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DOI:
10.1080/00268976.2012.695028
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发表时间:
2012-08
期刊:
影响因子:
1.7
通讯作者:
Xie XS
Xie XS
中科院分区:
化学4区
文献类型:
--
作者:
Lu FK;Ji M;Fu D;Ni X;Freudiger CW;Holtom G;Xie XS

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受激拉曼散射(SRS)显微镜通过探测样品中特定的拉曼活性分子振动模式,开辟了广泛的生化成像应用。然而,最初采用皮秒脉冲激发的方法只能实现单个拉曼波段的快速化学作图。在这里,我们提出了一种新的SRS显微技术,使用基于光栅的脉冲整形器进行激发和基于光栅的光谱仪进行检测,以实现高灵敏度和高采集速度的同时多色SRS成像。特别是,我们使用测量的CH2和CH3拉伸信号的线性组合来同时绘制蛋白质和脂质含量的分布。
Stimulated Raman scattering (SRS) microscopy has opened up a wide range of biochemical imaging applications by probing a particular Raman-active molecule vibrational mode in the specimen. However, the original implementation with picosecond pulse excitation can only realize rapid chemical mapping with a single Raman band. Here we present a novel SRS microscopic technique using a grating-based pulse shaper for excitation and a grating-based spectrograph for detection to achieve simultaneous multicolor SRS imaging with high sensitivity and high acquisition speeds. In particular, we used linear combination of the measured CH2 and CH3 stretching signals to map the distributions of protein and lipid contents simultaneously.
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